Yanda Bio delivers a robust and easy-to-use Horse IL-6 ELISA kit for the quantitative measurement of interleukin-6 in equine serum, plasma, cell culture supernatant, and tissue homogenates. As a developer of over 6,000 validated ELISA targets covering human, mouse, rat, horse, rabbit, canine, bovine, chicken, duck, sheep, monkey, fish, insect, plant, and microbial species, we are the trusted partner for more than 60 universities and research institutes across China. Take advantage of free shipping when you buy any 3 kits, and use our elisa testing service at no additional cost: our professional lab will perform the entire assay on your samples, record the complete procedure on video, and deliver all raw data. We also offer specialized immunogenicity elisa testing services for equine biologic development. If your study demands higher sensitivity, ask us about our NHS‑biotin‑enhanced ELISA format.
Why Measure Equine Interleukin-6?
Equine interleukin-6 (IL-6) is a multifunctional cytokine that acts as a pivotal mediator of the acute‑phase response, inflammation, and immune regulation. In horses, circulating IL-6 levels rise sharply during systemic inflammation caused by endotoxemia, surgical trauma, strenuous exercise, and infectious diseases such as strangles or colitis. IL-6 is also implicated in equine metabolic syndrome and laminitis, making it a key biomarker for monitoring disease severity and therapeutic response. An accurate horse IL-6 ELISA test or equine IL-6 quantitative assay empowers researchers to track pro‑inflammatory cascades, evaluate anti‑inflammatory drug candidates, and study the equine cytokine network with confidence. Whether you are performing a routine horse cytokine assay or a detailed equine interleukin-6 detection panel, this horse IL-6 immunoassay provides reproducible, publication‑ready data.
A Complete Service, Not Just a Kit
Yanda Bio goes beyond supplying reagents. Your purchase includes free, full‑service elisa testing service handled by experienced technicians in our dedicated laboratory. Simply send us your equine samples, and we will:
- Prepare standards, controls, and sample dilutions according to the protocol
- Run the ELISA plate with precise timing and thorough washing
- Record the entire experiment on video for complete transparency
- Provide raw OD values, a standard curve, and calculated concentrations in a clean Excel format
This elisa testing service eliminates hands‑on time, instrument dependency, and learning curves, making it ideal for labs new to equine biomarker quantification or those needing to process large sample batches rapidly. For drug development teams, our immunogenicity elisa testing services measure anti‑drug antibody responses against equine or equine‑derived therapeutic proteins with full documentation. Additionally, for analytes present at very low abundance, we supply an ultra‑sensitive version of this horse IL-6 ELISA kit that incorporates an NHS‑biotin amplification step. Read our technical resource: [NHS-Biotin in ELISA Kit: How It Amplifies Your Signal and How to Use It Correctly] (insert your link here) to learn how biotin‑streptavidin systems push detection limits even lower.
Assay Principle – Solid‑Phase Sandwich Immunoassay
This horse IL-6 ELISA kit is built on a solid‑phase sandwich ELISA format with a single incubation step. The microplate wells are pre‑coated with a highly specific capture antibody against equine IL-6. When you add standards or samples together with an HRP‑linked detection antibody, IL-6 is simultaneously captured and labeled in solution, forming an antibody‑antigen‑enzyme sandwich inside each well. After a thorough wash, the TMB substrate is introduced; the HRP drives a color change from blue to yellow upon acidic stopping. The absorbance at 450 nm increases linearly with the amount of equine IL-6 present, allowing concentration interpolation from a standard curve.
Kit Components
| Component | 96‑Well Format | 48‑Well Format | Remarks |
|---|---|---|---|
| Pre‑coated microplate | 12 strips × 8 wells | 12 strips × 4 wells | Ready to use |
| Standard (S0–S5) | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes | 0, 40, 80, 160, 320, 640 pg/mL |
| Sample Diluent | 6 mL | 3 mL | – |
| Detection Antibody‑HRP | 10 mL | 5 mL | – |
| 20× Wash Buffer | 25 mL | 15 mL | Dilute before use |
| Substrate A | 6 mL | 3 mL | – |
| Substrate B | 6 mL | 3 mL | – |
| Stop Solution | 6 mL | 3 mL | – |
| Plate Sealers | 2 sheets | 2 sheets | – |
| Instruction Manual | 1 copy | 1 copy | – |
| Self‑sealing Bag | 1 piece | 1 piece | – |
The zero standard (S0) serves as the blank/negative control.
Performance Characteristics
| Parameter | Specification |
|---|---|
| Detection Range | 1.0 – 480 pg/mL |
| Analytical Sensitivity | < 1.0 pg/mL |
| Standard Curve Range | 0 – 640 pg/mL |
| Accuracy | Standard linear regression R ≥ 0.9900 |
| Precision | Intra‑assay CV < 15%; Inter‑assay CV < 15% |
| Specificity | Recognizes native equine IL‑6; minimal cross‑reactivity with related cytokines |
| Validated Samples | Serum, plasma (EDTA/citrate/heparin), cell supernatant, tissue homogenate |
| Shelf Life | 6 months at 2–8°C, protected from light |
Sample Handling Guidelines
- Serum: Collect blood in endotoxin‑ and pyrogen‑free tubes. Let clot, centrifuge at 3000 rpm for 10 min, and separate the serum promptly. If your workflow includes cell lysis buffers, refer to our internal note on the effect of cell lysis buffer components on ELISA performance (insert your internal link).
- Plasma: Use EDTA, citrate, or heparin. Centrifuge at 3000 rpm for 30 min and harvest the supernatant.
- Cell Culture Supernatant: Spin at 3000 rpm for 10 min to remove debris.
- Tissue Homogenate: Homogenize equine tissue in normal saline, centrifuge at 3000 rpm for 10 min, and keep the supernatant.
- Storage: Aliquot and store at -20°C if not tested immediately. Avoid repeated freeze‑thaw cycles. Thaw fully at room temperature and mix gently before use.
Important: The assay protocol dilutes all samples 5‑fold. Multiply the calculated concentration by 5 to obtain the actual value.
At‑a‑Glance Assay Procedure
Full instructions are supplied with the kit. A condensed workflow is shown below.
- Equilibrate: Bring all reagents and strips to room temperature (20 min). Reseal unused strips and return to 4°C.
- Add Standards & Samples: Pipette 50 µL standards; for samples, 10 µL sample + 40 µL sample diluent. Leave blank wells without sample or standard.
- One‑Step Incubation: Add 100 µL HRP‑Detection Antibody to all wells except blanks. Cover and incubate at 37°C for 60 min.
- Wash: Aspirate, fill with 1× Wash Buffer (dilute 20× concentrate 1:20; dissolve crystals by warming), soak 1 min, then aspirate. Repeat 5 times. Blot dry.
- Substrate: Add 50 µL Substrate A + 50 µL Substrate B. Incubate in the dark at 37°C for 15 min.
- Stop & Read: Add 50 µL Stop Solution. Measure OD at 450 nm within 15 min. Generate the standard curve and compute concentrations, applying the 5× dilution factor.
Essential Equipment (not provided)
- Microplate reader (450 nm)
- Precision pipettes with tips (0.5‑10 µL, 2‑20 µL, 20‑200 µL, 200‑1000 µL)
- 37°C incubator or water bath
- Deionized water, graduated cylinders, absorbent paper
Key Operational Notes
- Wash buffer crystals are normal; warm to redissolve fully.
- The S0 standard serves as the blank/negative control.
- Calibrate pipettes regularly; if sample number is high, use a multichannel pipette to complete pipetting within 5 min.
- Run a duplicate standard curve with every experiment.
- For samples exceeding the highest standard, pre‑dilute further and multiply by the total dilution factor.
- Plate sealers are single‑use. Keep TMB away from light. Treat all specimens as biohazardous.
Pair with These Equine Cytokine ELISA Kits
Build a comprehensive equine inflammatory profile by combining this horse IL-6 assay with:
- Horse Tumor Necrosis Factor Alpha (TNF‑α) ELISA Kit – a key pro‑inflammatory cytokine in equine septic shock and chronic inflammation.
- Horse Interleukin‑1 Beta (IL‑1β) ELISA Kit – a master mediator of equine acute laminitis and osteoarthritis.
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Explore our growing catalog of equine ELISA kits for chemokines, growth factors, and acute‑phase proteins.
Why Researchers Trust Yanda Bio
- Extensive target portfolio: Over 6,000 ready‑to‑order ELISA kits across more than 15 species, from human and mouse to horse, zebrafish, and plant.
- Academic collaborations: Trusted by 60+ Chinese universities for long‑term research partnerships and custom development projects.
- Manufacturer‑direct advantages: Consistent lot‑to‑lot performance, competitive pricing, and responsive technical support.
- Risk‑free ordering: Buy 3 kits and receive free shipping.
- Complimentary testing service: Every kit purchase includes access to our elisa testing service — we run the assay, provide a video, and deliver all data at no extra cost.
- High‑sensitivity options: Need a lower detection limit? Ask about our NHS‑biotin amplified ELISA kits.
Disclaimer: This kit is for research use only. It is not intended for clinical diagnosis or veterinary therapeutic decision‑making. Strict adherence to the protocol is required; deviations are the responsibility of the experimenter. Yanda Bio assumes no liability for consequences arising from improper use.
Yanda Bio – Equipping equine research with precise ELISA tools, expert elisa testing service, and reliable immunogenicity elisa testing services.
