Yanda Bio offers a sensitive, one-step Horse PTH ELISA kit designed for the accurate quantification of equine parathyroid hormone (PTH, also known as parathormone) in serum, plasma, cell culture supernatant, and tissue homogenates. As a professional manufacturer with a product line of over 6,000 validated ELISA targets—spanning human, mouse, rat, horse, rabbit, canine, bovine, chicken, duck, sheep, monkey, fish, insect, plant, and microbial species—we support more than 60 universities and research institutes across China. Enjoy free shipping when you order any 3 kits, and take advantage of our complimentary elisa testing service: our experienced laboratory team will run the assay on your samples, provide a complete video recording of the procedure, and deliver all raw data at no extra cost. We also provide immunogenicity elisa testing services for preclinical drug development. For studies requiring ultra‑low detection, ask about our NHS‑biotin‑amplified ELISA format. This kit is validated for equine samples exclusively and must not be used with human specimens.
Why Parathyroid Hormone Is Critical in Equine Health
Parathyroid hormone (PTH) is an 84‑amino‑acid polypeptide secreted by the chief cells of the parathyroid glands. Acting as a master regulator of calcium homeostasis, PTH influences bone remodeling, renal calcium reabsorption, and intestinal calcium absorption in response to low serum Ca²⁺ levels. In horses, PTH dysregulation underlies several clinically significant disorders. Equine hyperparathyroidism, often secondary to dietary calcium deficiency or oxalate‑rich forages, leads to mobilization of skeletal calcium stores, bone resorption, and conditions such as nutritional secondary hyperparathyroidism (NSH, “big head disease”). Conversely, hypoparathyroidism can result in life‑threatening hypocalcemia, presenting as muscle fasciculation, tetany, and ileus. Paraneoplastic hypercalcemia driven by PTH‑related protein (PTHrP) is another important differential in equine medicine.
Quantifying PTH with an equine PTH ELISA kit gives researchers and veterinary investigators a direct window into calcium‑phosphorus balance, bone turnover, and parathyroid gland function. Whether you are monitoring metabolic bone disease, studying the effects of intensive exercise on calcium metabolism, or evaluating dietary interventions in growing foals, this horse parathyroid hormone immunoassay provides reproducible, trustworthy data.
Free ELISA Testing Service – We Run Your Samples
Your purchase of the Horse PTH ELISA kit includes more than reagents. Yanda Bio’s professional laboratory provides a free elisa testing service that eliminates the burden of in‑house assay optimization. Simply send us your prepared equine samples, and our technicians will:
- Handle sample thawing, aliquoting, and plate layout
- Perform the complete assay with precise incubation, washing, and detection steps
- Record the entire experiment on video for complete procedural transparency
- Compile raw OD values, standard curve data, and final PTH concentrations in a clean Excel file
This service is especially advantageous for laboratories that lack ELISA‑specific equipment or need high‑throughput sample processing without training additional personnel. For equine pharmaceutical and vaccine development programs, Yanda Bio also offers specialized immunogenicity elisa testing services that measure anti‑drug antibody responses and cytokine release profiles, all with thorough documentation. Whether you need a single plate analyzed or a large‑scale screening, our elisa testing service delivers expert‑level results and saves you valuable time.
Assay Principle – One‑Step Sandwich Immunoassay
The horse PTH ELISA kit utilizes a one‑step solid‑phase sandwich ELISA method. Microplate wells are pre‑coated with a high‑affinity capture antibody specific for equine PTH. During the assay, standards or samples are added together with an HRP‑linked detection antibody. In a single 60‑minute incubation, PTH molecules are simultaneously captured onto the well surface and labeled with HRP, forming a stable antibody‑antigen‑enzyme sandwich. After rigorous washing to remove unbound material, the TMB substrate is introduced. The HRP catalyzes a color change from blue to yellow upon acid stopping; the absorbance at 450 nm is directly proportional to the PTH concentration in the sample. Unknown concentrations are calculated by interpolation from a six‑point standard curve.
Kit Components
| Component | 96‑Well Format | 48‑Well Format | Remarks |
|---|---|---|---|
| Pre‑coated Microplate | 12 strips × 8 wells | 12 strips × 4 wells | Ready to use |
| Standard (S0–S5) | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes | Concentrations: 0, 40, 80, 160, 320, 640 pg/mL |
| Sample Diluent | 6 mL | 3 mL | – |
| Detection Antibody‑HRP | 10 mL | 5 mL | – |
| 20× Wash Buffer | 25 mL | 15 mL | Dilute to 1× before use |
| Substrate A | 6 mL | 3 mL | – |
| Substrate B | 6 mL | 3 mL | – |
| Stop Solution | 6 mL | 3 mL | – |
| Plate Sealers | 2 sheets | 2 sheets | – |
| Instruction Manual | 1 copy | 1 copy | – |
| Self‑sealing Bag | 1 piece | 1 piece | For storing unused strips |
S0 (0 pg/mL) serves as the blank/negative control.
Assay Performance Specifications
| Parameter | Specification |
|---|---|
| Detection Range | 1.0 – 120 pg/mL |
| Analytical Sensitivity | < 1.0 pg/mL |
| Accuracy | Standard curve R ≥ 0.9900 |
| Precision | Intra‑assay CV < 6%; Inter‑assay CV < 9% |
| Specificity | Recognizes equine PTH; negligible cross‑reactivity with PTHrP or other related peptides |
| Sample Types Validated | Equine serum, plasma (EDTA/citrate/heparin), cell culture supernatant, tissue homogenate |
| Shelf Life | 6 months at 2–8°C, protected from light |
Sample Collection & Preparation
Proper sample handling is essential for reliable PTH measurement.
- Serum: Collect blood in endotoxin‑ and pyrogen‑free tubes, allow to clot, and centrifuge at 3000 rpm for 10 min. Separate serum promptly.At this point, it is important to select the appropriate cell lysis solution. If you are unsure, you can consult us.<How Cell Lysis Buffers Impact Your ELISA Results (And How to Avoid Costly Mistakes)>
- Plasma: Use EDTA, citrate, or heparin as anticoagulant. Centrifuge at 3000 rpm for 30 min and collect the supernatant.
- Cell Culture Supernatant: Centrifuge at 3000 rpm for 10 min to remove particulates.
- Tissue Homogenate: Homogenize equine tissue (e.g., parathyroid, bone) in normal saline, spin at 3000 rpm for 10 min, and retain the supernatant.
- Storage: If not assayed immediately, aliquot and store at -20°C. Avoid repeated freeze‑thaw cycles. Thaw completely at room temperature and mix gently before use.
Crucial species limitation: This equine PTH ELISA has been validated exclusively for horse samples. It must not be applied to human specimens, as the antibodies and standard curve are optimized for equine PTH and will not provide accurate data on human material. Any use with human samples is outside the intended research purpose and may lead to invalid results.
Condensed Assay Protocol
Detailed, step‑by‑step instructions are provided with each kit. Below is a quick‑reference overview.
- Equilibration: Bring all reagents and the required number of pre‑coated strips to room temperature for 20 min. Reseal and refrigerate unused strips immediately.
- Standard & Sample Loading: Pipette 50 µL of each standard into designated wells. For sample wells, add 10 µL sample + 40 µL sample diluent (final 5‑fold dilution). Blank wells receive neither sample nor standard.
- One‑Step Incubation: Add 100 µL HRP‑Detection Antibody to all wells except blanks. Cover with a plate sealer and incubate at 37°C for 60 min.
- Washing: Aspirate, then wash 5 times with 1× wash buffer (dilute 20× concentrate 1:20; dissolve any crystals by warming). Soak 1 min per wash, then blot dry.
- Substrate: Add 50 µL Substrate A + 50 µL Substrate B. Incubate in the dark at 37°C for 15 min.
- Stop & Read: Add 50 µL Stop Solution. Measure OD at 450 nm within 15 min. Plot the standard curve and calculate sample concentrations. Multiply the result by 5 to correct for the assay dilution.
Required Equipment (not included)
- Microplate reader (450 nm)
- Precision pipettes (0.5‑10, 2‑20, 20‑200, 200‑1000 µL)
- 37°C incubator or water bath
- Deionized water, graduated cylinders, absorbent paper
Key Technical Notes
- Wash buffer crystals are normal; dissolve by warming before dilution.
- The S0 zero standard is the blank/negative control.
- Use calibrated pipettes; a multichannel pipette is recommended for large sample numbers to complete pipetting within 5 min.
- Run a fresh standard curve in duplicate with every experiment.
- If a sample OD exceeds the highest standard, pre‑dilute further and multiply by the additional factor.
- Plate sealers are for single use. Protect TMB from light. Treat all specimens as potentially biohazardous.
Related Equine Calcium & Bone Metabolism ELISA Kits
To build a comprehensive picture of calcium homeostasis and skeletal remodeling in the horse, pair this horse PTH ELISA kit with the following assays:
- Horse Interleukin-8(IL-8) ELISA Kit – For quantitative measurement of interleukin-6 in equine serum, plasma, cell culture supernatant, and tissue homogenates.
- Horse Osteocalcin (Bone Gla Protein) ELISA Kit – a marker of osteoblast activity and bone formation, helpful for monitoring bone turnover in growing foals or during orthopedic disease.
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Explore our wider equine portfolio for vitamin D metabolites, bone‑specific alkaline phosphatase, and other metabolic markers.
Why Researchers Choose Yanda Bio
- Extensive catalog: Over 6,000 ELISA targets validated across human, equine, rodent, livestock, companion animal, aquatic, and plant species.
- Academic collaborations: Long‑standing partnerships with 60+ universities and research institutions in China, supporting both routine and custom ELISA development.
- Manufacturer‑direct benefits: Consistent lot‑to‑lot performance, competitive pricing, and responsive technical support.
- Free shipping: Order any 3 kits and enjoy complimentary delivery.
- Complimentary elisa testing service: We run the assay for you at no charge—full video recording and all data provided.
- High‑sensitivity options: Need to detect very low PTH concentrations? Inquire about our NHS‑biotin‑enhanced ELISA kits for improved signal amplification.
- Immunogenicity testing: Our immunogenicity elisa testing services support preclinical drug and vaccine evaluation with robust anti‑drug antibody assays.
Disclaimer: This kit is for research use only. It is not intended for clinical diagnosis or veterinary therapeutic decision‑making. This equine PTH ELISA kit is validated for equine samples exclusively and must not be used with human specimens. Follow the provided protocol precisely; any deviations are the responsibility of the experimenter. Yanda Bio assumes no liability for consequences arising from improper use.
Yanda Bio – Supplying high‑quality equine ELISA kits, plus expert elisa testing service and immunogenicity elisa testing services, to advance your endocrine and bone metabolism research with precision and convenience.
