Yanda Bio offers a precise, ready-to-use Human Estradiol (E2) ELISA kit for the quantitative determination of 17β‑estradiol—the most potent natural estrogen—in human serum, plasma, cell culture supernatant, and tissue homogenates. Backed by a portfolio of over 6,000 validated ELISA targets across human, mouse, rat, horse, rabbit, canine, bovine, chicken, duck, sheep, monkey, fish, insect, plant, and microbial species, we support more than 60 universities and research institutes in China. Our kits are renowned for high reproducibility, strong specificity, and excellent sensitivity—with custom sensitivity configurations available upon request. Take advantage of free shipping on any 3 kits, and leverage our complimentary elisa testing service: our expert laboratory team will run the assay on your samples, record the entire procedure on video, and deliver all raw data at no additional charge. For studies that demand ultra‑low detection limits, ask about our NHS‑biotin‑enhanced ELISA kits. We also provide rigorous immunogenicity elisa testing services to support preclinical drug and vaccine development. This kit is validated for human specimens exclusively.
Why Measure Estradiol (E2)?
Estradiol (E2, 17β‑estradiol) is the principal female sex hormone and a critical regulator of reproductive function, bone density, lipid metabolism, cardiovascular health, and cognitive processes. Circulating E2 levels fluctuate across the menstrual cycle, decline sharply at menopause, and are altered in various pathological states. In research settings, accurate quantification of estradiol is indispensable for:
- Reproductive endocrinology studies: tracking follicular development, ovulation, and luteal function.
- Breast cancer and hormone‑dependent tumor research: E2 drives the proliferation of estrogen receptor‑positive tumors, and measuring E2 helps evaluate aromatase inhibitor efficacy, endocrine resistance, and the intratumoral estrogen environment.
- Osteoporosis and skeletal metabolism: E2 is a key regulator of bone remodeling, and its deficiency leads to accelerated bone loss.
- Drug development and clinical trials: assessing the impact of novel therapeutics, endocrine disruptors, or hormone replacement regimens on endogenous estradiol concentrations.
Whether you need a reliable human estradiol assay, an E2 quantitative ELISA, a 17β‑estradiol detection kit, or a human estrogen hormone immunoassay, this kit delivers sensitive, reproducible data for your translational and preclinical investigations.
Assay Principle – One‑Step Sandwich ELISA
This Human Estradiol (E2) ELISA kit employs a validated double‑antibody one‑step sandwich enzyme‑linked immunosorbent assay. Microplate wells are pre‑coated with an antibody specific for estradiol (E2) . Standards and samples are added together with an HRP‑conjugated detection antibody, forming an antibody‑antigen‑enzyme sandwich in a single 60‑minute incubation. After thorough washing to remove unbound material, TMB substrate is introduced. The HRP catalyzes a color change from blue to yellow upon acid stopping; the absorbance at 450 nm is directly proportional to the concentration of estradiol (E2) . Sample concentrations are calculated by interpolation from a six‑point standard curve.
Kit Components
| Component | 96‑Well Format | 48‑Well Format | Remarks |
|---|---|---|---|
| Pre‑coated Microplate | 12 strips × 8 wells | 12 strips × 4 wells | Coated with anti‑E2 antibody |
| Standard (S0–S5) | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes | Concentrations: 0, 40, 80, 160, 320, 640 pg/mL |
| Sample Diluent | 6 mL | 3 mL | – |
| Detection Antibody‑HRP | 10 mL | 5 mL | – |
| 20× Wash Buffer | 25 mL | 15 mL | Dilute to 1× before use |
| Substrate A | 6 mL | 3 mL | – |
| Substrate B | 6 mL | 3 mL | – |
| Stop Solution | 6 mL | 3 mL | – |
| Plate Sealers | 2 sheets | 2 sheets | – |
| Instruction Manual | 1 copy | 1 copy | – |
| Self‑sealing Bag | 1 piece | 1 piece | For unused strip storage |
The S0 zero standard (0 pg/mL) serves as the blank/negative control.
Performance Characteristics
| Parameter | Specification |
|---|---|
| Detection Range | 5 – 240 pmol/L |
| Analytical Sensitivity | < 1.0 pmol/L |
| Accuracy | Standard curve R ≥ 0.9900 |
| Precision | Intra‑assay CV < 15%; Inter‑assay CV < 15% |
| Specificity | Recognizes human estradiol; no cross‑reactivity with structurally related steroids (estrone, estriol, progesterone, testosterone) |
| Validated Sample Types | Human serum, plasma (EDTA/citrate/heparin), cell culture supernatant, tissue homogenate |
| Shelf Life | 6 months at 2–8°C, protected from light |
Need even greater sensitivity? Yanda Bio provides custom‑tuned sensitivity and NHS‑biotin‑amplified ELISA kits for ultra‑low estradiol detection. Contact our technical team for details.
Free ELISA Testing Service – We Run Your Samples
Your kit purchase includes access to Yanda Bio’s free elisa testing service. Simply send us your human samples, and our dedicated laboratory professionals will:
- Handle all sample preparation, plate layout, and quality control
- Run the complete ELISA with precise incubation, washing, and detection
- Video‑record the entire experimental workflow for full transparency
- Compile raw OD data, the standard curve, and final E2 concentrations in a clearly formatted Excel file
This service eliminates the need for in‑house optimization and instrument maintenance, making it especially valuable for high‑throughput hormone profiling or drug‑response studies. We also offer standalone immunogenicity elisa testing services for teams developing biologics, biosimilars, or therapeutic antibodies, delivering robust anti‑drug antibody measurement with full documentation.
Sample Collection, Processing & Storage
- Serum: Collect blood in endotoxin‑ and pyrogen‑free tubes, allow to clot undisturbed, centrifuge at 3000 rpm for 10 min, and promptly separate the serum.
- Plasma: Use EDTA, citrate, or heparin as anticoagulant. Centrifuge at 3000 rpm for 30 min and harvest the supernatant.
- Cell Culture Supernatant: Spin at 3000 rpm for 10 min to remove particulates and debris.
- Tissue Homogenate (e.g., breast tumor, ovarian, placental): Homogenize in normal saline, centrifuge at 3000 rpm for 10 min, and retain the supernatant.
- Storage: If not assayed immediately, aliquot and store at -20°C. Avoid repeated freeze‑thaw cycles. Thaw completely at room temperature and mix gently before use.
Sample dilution note: During the assay, samples are diluted 5‑fold. Multiply the obtained concentration by 5 to obtain the actual sample value.
Condensed Assay Protocol
Detailed instructions are included with each kit. Below is a quick reference.
- Equilibrate: Allow all reagents and the required number of pre‑coated strips to reach room temperature (20 min). Reseal and refrigerate unused strips.
- Load: Pipette 50 µL of each standard into designated wells. For sample wells, add 10 µL sample + 40 µL sample diluent (5‑fold dilution). Leave blank wells empty.
- One‑Step Incubation: Add 100 µL HRP‑Detection Antibody to all wells except blanks. Cover with a plate sealer and incubate at 37°C for 60 min.
- Washing: Aspirate the liquid, fill each well with 1× wash buffer (dilute 20× concentrate; dissolve any crystals by warming), soak for 1 min, then aspirate. Repeat for a total of 5 washes. Blot dry.
- Substrate: Add 50 µL Substrate A and 50 µL Substrate B. Incubate in the dark at 37°C for 15 min.
- Stop & Read: Add 50 µL Stop Solution. Read OD at 450 nm within 15 min. Generate the standard curve and calculate concentrations. Apply the 5× dilution correction.
Required Equipment (not provided)
- Microplate reader (450 nm)
- Precision pipettes (0.5‑10, 2‑20, 20‑200, 200‑1000 µL)
- 37°C incubator or water bath
- Deionized water, graduated cylinders, absorbent paper
Key Technical Notes
- Wash buffer crystals are normal; dissolve by warming before dilution.
- S0 standard serves as blank/negative control.
- Use calibrated pipettes; a multichannel pipette is recommended for large sample numbers.
- Run a fresh duplicate standard curve with each experiment.
- If a sample OD exceeds the highest standard, pre‑dilute further and multiply by the total dilution factor.
- Plate sealers are single‑use. Protect TMB from light. Treat all human biospecimens as potentially biohazardous.
Expand Your Estrogen Profiling Panel
Pair this human estradiol ELISA kit with the following assays to build a comprehensive estrogen metabolism analysis:
- Human Estrone (E1) ELISA Kit – quantify the second major circulating estrogen; together with E2, it provides a full picture of aromatase pathway activity.
- Human Estriol (E3) ELISA Kit – the dominant estrogen of pregnancy; useful for placental function studies and fetal‑placental endocrinology.
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Explore our expanding catalog of steroid hormone, SHBG, and estrogen conjugate ELISA kits for multi‑analyte endocrine research.
Why Researchers Partner with Yanda Bio
- Exceptional catalog: Over 6,000 validated ELISA targets covering human, animal, and plant species.
- Quality you can trust: Kits are rigorously tested for high reproducibility, strong specificity, and excellent sensitivity, with custom sensitivity available.
- Academic collaborations: More than 60 Chinese universities and research institutes rely on our products and custom development services.
- Free shipping: Order any 3 kits and enjoy complimentary delivery.
- Complimentary elisa testing service: We run the assay on your samples, record the process on video, and provide complete raw data at no extra cost.
- High‑sensitivity formats: NHS‑biotin‑amplified ELISA kits available for ultra‑low estradiol concentrations.
- Immunogenicity testing: Our immunogenicity elisa testing services provide rigorous anti‑drug antibody assessment for preclinical programs.
Disclaimer: This kit is for research use only. It is not intended for clinical diagnosis or therapeutic decision‑making. For clinical evaluation of hormonal status, dedicated estradiol clinical assays should be used. This human estradiol ELISA kit is validated for human samples exclusively and is not designed for cross‑species application. Follow the provided protocol precisely; any deviations are the responsibility of the experimenter. Yanda Bio shall not be liable for consequences arising from improper use.
Yanda Bio – Advanced ELISA kits, reliable elisa testing service, and specialized immunogenicity elisa testing services to support your reproductive endocrinology and hormone‑dependent disease research.
