Product Overview: Why Measure Mouse IL‑6?
Interleukin 6 (IL‑6) is a pleiotropic pro‑inflammatory cytokine produced by T cells, macrophages, fibroblasts, and endothelial cells. It plays a central role in acute phase response, immune regulation, hematopoiesis, and inflammation. Dysregulated IL‑6 expression is associated with rheumatoid arthritis, inflammatory bowel disease, cancer, cachexia, and sepsis. Accurate quantification of Mouse IL‑6 is essential for:
- Inflammation and autoimmune disease research using murine models
- Cytokine storm and sepsis studies
- Cancer immunology and tumor microenvironment investigations
- Drug development targeting IL‑6/IL‑6R pathways
This kit is a custom‑designed ELISA test kit validated for mouse serum, plasma, and cell culture samples, offering reliable performance for preclinical research.
Why Yanda Bio is Your Trusted ELISA Test Kit Manufacturer & elisa diagnostic kit supplier
| EEAT Factor | Our Commitment |
|---|---|
| Experience | 6,000+ factors developed; 60+ university collaborations; years of expertise in cytokine assays. |
| Expertise | Proprietary antibody pairs validated for mouse IL‑6; sandwich ELISA ensures high specificity and minimal cross‑reactivity with other mouse interleukins (IL‑1, IL‑10, TNF‑α, etc.). |
| Authoritativeness | Kits used in academic publications; ISO‑aligned manufacturing; batch‑to‑batch consistency. |
| Trustworthiness | Rapid custom development (3–7 days); transparent specifications; unconditional quality guarantee; free shipping on 3+ kits. |
Superior Quality Metrics
- Intra‑assay CV < 5 % — excellent within‑run precision
- Inter‑assay CV < 8 % — outstanding run‑to‑run consistency
- High specificity — no cross‑reactivity with mouse IL‑1β, IL‑2, IL‑4, IL‑10, IFN‑γ, or TNF‑α
Kit Components: What’s Inside (96T Configuration)
| Component | Quantity |
|---|---|
| Microplate (Pre‑coated) | 8 wells × 12 strips |
| Standards (lyophilised or liquid) | 0.3 mL × 6 tubes |
| Sample Diluent | 6 mL |
| HRP‑Conjugated Detection Antibody | 6 mL |
| 20× Wash Buffer | 25 mL |
| Substrate A | 6 mL |
| Substrate B | 6 mL |
| Stop Solution | 6 mL |
| Plate Sealer | 2 sheets |
| Manual | 1 copy |
48‑well configuration (8 wells × 6 strips) and bulk volumes available on request.
Assay Principle: Sandwich ELISA
This kit employs a solid‑phase sandwich enzyme‑linked immunosorbent assay. The microplate is pre‑coated with a monoclonal antibody specific for Mouse IL‑6. Standards and samples are added, allowing IL‑6 to bind to the immobilised antibody. After washing, a Horseradish Peroxidase (HRP)‑conjugated detection antibody is added, forming an antibody‑antigen‑antibody “sandwich”. Following a second wash, TMB substrate is added. The HRP enzyme catalyses a blue colour that turns yellow upon acidification. The optical density (OD) at 450 nm is directly proportional to the IL‑6 concentration in the sample.
Sample Dilution Note: For serum or plasma, samples are pre‑diluted 5‑fold during the procedure (10 μL sample + 40 μL diluent). Multiply final results by 5.
Sample Collection & Preparation
| Sample Type | Collection & Processing |
|---|---|
| Serum | Use pyrogen/endotoxin‑free tubes. Avoid cell stimulation. Centrifuge at 3000 rpm for 10 minutes. |
| Plasma | Use EDTA, citrate or heparin anticoagulant. Centrifuge at 3000 rpm for 30 minutes. |
| Cell Supernatant | Centrifuge at 3000 rpm for 10 minutes to remove particles. |
| Tissue Homogenate | Homogenise tissue in PBS (1:9 w/v), centrifuge at 5000×g for 10 minutes at 2–8 °C. Collect supernatant. |
| Storage | Extract as soon as possible. Store at –20 °C (≤ 1 month) or –80 °C (≤ 2 months). Avoid repeated freeze‑thaw cycles. |
Important: Do not use samples containing sodium azide (NaN₃), as it inhibits HRP activity.
Materials Required But Not Provided
- Microplate reader (450 nm)
- Precision pipettes and tips (0.5–10 μL, 2–20 μL, 20–200 μL, 200–1000 μL)
- 37 °C incubator or water bath
- Distilled or deionised water
Procedure Notes & Important Tips
- Bring all reagents to room temperature before use. Concentrated wash buffer may crystallise; dissolve crystals in a warm water bath.
- Unused strips must be resealed with desiccant and stored at 2–8 °C.
- Standard dilution: Prepare serial dilutions of the stock standard according to the lot‑specific datasheet.
- Sample addition: Add 40 μL sample diluent to each sample well, then 10 μL test sample. Mix gently without touching the well bottom.
- Incubation: Use the plate sealer and incubate at 37 °C for 60 minutes (first incubation).
- Washing: After each incubation, discard liquid, fill wells with wash buffer, stand for 30 seconds, then discard. Repeat 5 times. Incomplete washing may cause false positives.
- Substrate: Add Substrate A then B. Incubate in the dark at 37 °C for 10–15 minutes.
- Read OD: Add stop solution and read at 450 nm within 15 minutes.
Detailed Assay Procedure
- Remove required strips from the foil pouch after 20 min equilibration. Reseal unused strips.
- Standards: Add 50 μL of each standard concentration to standard wells.
- Samples: Add 40 μL sample diluent to sample wells, then 10 μL test sample. Blank wells: leave empty.
- Add 100 μL HRP‑conjugated detection antibody to all wells except blanks. Seal and incubate at 37 °C for 60 min.
- Aspirate, wash 5 times (manual or automatic washer).
- Add 50 μL Substrate A and 50 μL Substrate B to each well. Gently tap to mix. Incubate at 37 °C in the dark for 10–15 min.
- Add 50 μL stop solution. Read OD at 450 nm within 15 min.
Data Analysis
Plot standard curve: OD values (y‑axis) vs. standard concentrations (x‑axis) using linear regression (R ≥ 0.9900). Calculate sample concentrations from the curve. For serum/plasma samples, multiply by the dilution factor (5×). For cell culture supernatants or other matrices, use the appropriate dilution factor as validated.
Performance Specifications
| Parameter | Performance |
|---|---|
| Accuracy | Standard curve linear regression coefficient (R) ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 10 pg/mL |
| Detection Range | 32 – 2000 pg/mL |
| Specificity | No significant cross‑reactivity with mouse IL‑1α, IL‑1β, IL‑2, IL‑4, IL‑10, IFN‑γ, TNF‑α, or other cytokines |
| Intra‑assay CV | < 5 % |
| Inter‑assay CV | < 8 % |
| Storage | 2–8 °C, protect from light |
| Shelf Life | 6 months |
Pricing & Bulk Discounts
| Quantity | Price per Kit |
|---|---|
| 1–2 kits | Regular retail – contact us for quote |
| 3 or more kits | Wholesale price – standard kits available under $100 per kit |
| Custom orders | Quote based on volume and specifications |
Free shipping on all orders of three or more ELISA kits. All products are shipped with free door‑to‑door delivery (tax included).
Free sample testing service is available upon request – contact our sales team for details.
Related Products (Recommended for Cytokine Profiling)
Expand your inflammation research with these trusted ELISA kits from Yanda Bio:
- Porcine Interleukin 28B (IL-28B) ELISA Kit – for swine antiviral immunity studies
- Rabbit Interleukin 13 (IL-13) ELISA Kit – for Th2 immune response in rabbit models
- Mouse Interleukin 10 (IL-10) ELISA Kit – an anti‑inflammatory cytokine for murine immunology (available as custom order)
Using mouse IL‑6 together with IL‑10 and other Th1/Th2 markers provides a comprehensive view of immune regulation in murine models.
Why Choose Yanda Bio for Your Customed ELISA Test Kit Needs?
- Manufacturer direct – over 6,000 detectable targets, 60+ university partners
- Rapid custom development – 3–7 days turnaround for new targets or matrices
- Strict quality control – intra‑assay CV < 5 %, inter‑assay CV < 8 %
- Cost‑effective – regular kit wholesale price < $100 for volumes of 3+
- Free technical support – from sample collection to data analysis
- Free sample testing service – for bulk orders
As a leading ELISA test kit manufacturer and reliable elisa diagnostic kit supplier, Yanda Bio supports your research with high‑quality, affordable immunoassays across all major species, including mouse, rat, human, rabbit, porcine, monkey, horse, chicken, and more.
Disclaimer
Strict adherence to the protocol is required. Yanda Bio is not responsible for results obtained from deviations. This product is for research use only; not for clinical diagnosis or human use. The user assumes all responsibility for any consequences arising from misuse.
Ordering & Contact Information
To order this Mouse Interleukin 6 (IL-6) ELISA Kit (a reliable Customed ELISA Test Kit for murine inflammation studies), or to inquire about volume discounts, custom development, and format selection, please contact our sales team.
