Rat Interleukin 22 (IL-22) ELISA Kit

Interleukin-22 (IL-22) is a critical cytokine that shapes immune response and tissue repair, often acting as a key inflammatory factor in infection, autoimmune disease, and barrier defense. Accurately measuring this mediator is essential for preclinical research. Yanda Bio, a professional ELISA kit manufacturer with over 6,000 detectable targets, delivers a validated Rat Interleukin 22 (IL-22) ELISA Kit that combines reliable quantitative performance with the consistency demanded by long-term studies. As a dedicated inflammatory factor ELISA kit, it is engineered to help you capture subtle changes in rat serum, plasma, urine, cell culture supernates, and tissue homogenates.

Backed by ongoing collaborations with more than 60 Chinese universities, each Yanda Bio kit is produced under strict quality control to guarantee stable reactivity and high specificity. Our plates use a special high-binding surface treatment – the bottom of each well is modified to strongly adsorb proteins during antibody and antigen coating, giving you a stronger signal, better linearity, and lower well-to-well variability.

Key Features at a Glance

• Sensitive quantitative range: 0.4 ng/L – 25 ng/L
• Reliable sandwich assay format with high-binding pre-coated microplate
• Rigorous validation: intra-assay CV < 9%, inter-assay CV < 11%, linearity R ≥ 0.990
• Broad sample compatibility: serum, plasma, urine, cell culture supernate, and tissue homogenates
• Enhanced plate technology: specially treated surface for optimal antigen/antibody adsorption
• Quality you can trust: developed and manufactured by a producer supporting over 6,000 factors and maintaining long-term academic partnerships
• Buy more, save more: free shipping when you order any 3 ELISA kits

Intended Use

This kit is designed for the quantitative measurement of rat interleukin-22 (IL-22) in serum, plasma, urine, cell culture supernate, and related liquid samples. It is intended for research use only.

Detection Range

0.4 ng/L – 25 ng/L
Samples with IL-22 levels above the highest standard should be diluted with sample diluent before testing. Multiply the final result by the corresponding dilution factor.

Test Principle

This Rat Interleukin 22 (IL-22) ELISA Kit employs a double-antibody sandwich technique. A monoclonal antibody specific for rat IL-22 is pre-coated onto the high-binding microplate. Standards and samples are added, allowing IL-22 to bind to the immobilized antibody. After washing, an HRP-conjugated detection antibody that recognizes rat IL-22 is added, forming an antibody-antigen-enzyme complex. Unbound conjugate is removed by thorough washing. TMB substrate is then added; the HRP enzyme converts TMB into a blue product, which turns yellow after the stop solution is introduced. Absorbance is read at 450 nm. The color intensity is directly proportional to the concentration of rat IL-22 in the sample.

Kit Performance

• Linearity: correlation coefficient R ≥ 0.990 when comparing expected vs. measured concentrations of diluted samples
• Precision: intra-assay coefficient of variation (CV) < 9%; inter-assay CV < 11%
• Specificity: high specificity for rat IL-22, with negligible cross-reactivity to structurally related cytokines when used as directed

Sample Collection and Preparation

Proper sample handling preserves analyte integrity. Follow the guidance below; for full details, refer to the kit manual.

Serum – Allow blood to clot naturally at room temperature for 10–20 minutes. Centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant promptly. If precipitate appears after storage, centrifuge again before use.

Plasma – Use EDTA or sodium citrate as the anticoagulant as recommended for your study. Mix gently for 10–20 minutes after collection, then centrifuge at 2000–3000 rpm for 20 minutes. Transfer the supernatant and re-centrifuge if any precipitate forms.

Urine – Collect aseptically in a sterile tube. Centrifuge at 2000–3000 rpm for 20 minutes. Use the supernatant. The same approach applies to cerebrospinal fluid and pleural/ascitic fluid.

Cell culture supernate – For secreted proteins, collect the culture medium and centrifuge at 2000–3000 rpm for 20 minutes. For intracellular IL-22, resuspend cells in PBS (pH 7.2–7.4) at approximately 1×10⁶ cells/mL, lyse by repeated freeze-thaw cycles, then centrifuge and harvest the supernatant.

Tissue homogenates – Weigh the tissue, add a measured volume of PBS (pH 7.4), and homogenize thoroughly on ice. Centrifuge at 2000–3000 rpm for 20 minutes, then collect the supernatant. Aliquots can be frozen at -20°C or below for later use.

Critical notes:

• Extract and assay samples as soon as possible. If a delay is unavoidable, store at -20°C and avoid repeated freeze-thaw cycles.
• Do not use samples containing NaN₃. Sodium azide inhibits HRP activity and will cause inaccurate readings.

Reagent Preparation

• Bring all reagents to room temperature (15–30 minutes) before use.
• Wash buffer: If crystals appear in the concentrated wash buffer, warm the solution in a water bath until the crystals dissolve. Dilute the concentrate with distilled water (30-fold for the 96T format, 20-fold for the 48T format) to prepare 1× working wash buffer.
• Standards: Reconstitute as instructed in the manual and prepare serial dilutions to obtain the standard curve points: 24 ng/L, 12 ng/L, 6 ng/L, 3 ng/L, and 1.5 ng/L.

Assay Procedure Summary

1. Add standards and samples: Pipette 50 µL of each standard into designated wells. For sample wells, first add 40 µL of sample diluent, then 10 µL of sample (5-fold final dilution). Leave blank wells empty of sample and enzyme conjugate.
2. Incubate: Cover the plate with a fresh plate sealer and incubate at 37°C for 30 minutes.
3. Wash: Remove the sealer, discard the liquid, and wash each well 5 times with 1× wash buffer. Soak for 30 seconds per wash, then blot dry.
4. Add enzyme conjugate: Add 50 µL of HRP-conjugated detection reagent to each well, except the blanks.
5. Incubate: Cover and incubate again at 37°C for 30 minutes.
6. Wash: Repeat the 5-step wash process.
7. Develop color: Add 50 µL of Chromogen Solution A and 50 µL of Chromogen Solution B to each well. Mix gently and incubate in the dark at 37°C for 10 minutes.
8. Stop reaction: Add 50 µL of stop solution to each well. The color changes from blue to yellow.
9. Measure absorbance: Read the optical density (OD) at 450 nm within 15 minutes, using the blank well to zero the reader.

Calculation of Results

Construct a standard curve by plotting the OD values against the known concentrations of the standards (24, 12, 6, 3, 1.5 ng/L). Use the resulting linear regression equation or curve to determine the IL-22 concentration of each sample. Multiply the obtained value by the final dilution factor (e.g., ×5) to calculate the actual concentration. If the sample was further diluted beforehand, also multiply by that extra factor.

Storage and Stability

• Storage: 2–8°C for all kit components.
• Shelf life: 6 months from the date of manufacture when stored unopened as directed.
• After opening the pre-coated plate, return unused strips to the sealed pouch with desiccant and store at 2–8°C.

Important Precautions

• Equilibrate the kit to room temperature for 15–30 minutes before opening.
• Use a calibrated pipette for all steps; perform the entire addition within 5 minutes when possible. A multichannel pipette is recommended for large sample numbers.
• Plate sealers are single-use only to prevent cross-contamination.
• Protect the TMB substrate from light.
• If a sample OD exceeds that of the highest standard, re-test after diluting the sample appropriately with sample diluent.
• Do not mix components from different kit lots.
• This kit detects native IL-22; samples containing NaN₃ are incompatible.
• Follow the protocol strictly; results must be determined using a microplate reader.
• Treat all specimens, wash buffers, and waste as potentially infectious.

Why Choose Yanda Bio for Your Inflammatory Factor ELISA Kit Needs

Yanda Bio is not just a supplier – we are a specialized ELISA kit manufacturer trusted by over 60 universities across China. Our portfolio spans more than 6,000 targets, encompassing cytokines, chemokines, and a wide array of inflammatory factor ELISA kits. Each kit is built around a high-binding microplate with a specially engineered surface to maximize protein adsorption, so you see strong signals even at low analyte concentrations. The outcome: stable lot-to-lot performance, high specificity, and the kind of reproducibility that powers credible publications.

Free shipping on every 3 kits – mixing different targets is welcome, making it easy to build your own inflammation panel.

Related Inflammatory Factor ELISA Kits You May Need

To complement your IL-22 study, consider these two equally robust inflammatory factor ELISA kits from Yanda Bio (internal links can be added):

• Mouse IL-6 ELISA Kit – Interleukin-6 is a quintessential inflammatory factor driving acute phase responses and chronic inflammation. This kit delivers the same high-binding plate advantage and quantitative precision for mouse serum and plasma.
• Rat TNF-α ELISA Kit – Tumor necrosis factor-alpha sits at the apex of the inflammatory cascade. Our TNF-α kit offers excellent specificity and sensitivity, making it a reliable choice for mechanistic and interventional studies.

Both kits share the same rigorous quality framework, substrate system, and plate technology as your Rat Interleukin 22 (IL-22) ELISA Kit, ensuring seamless multiplexing-like workflows when you assess multiple biomarkers in parallel.

Ordering Information

• Cat. No.: (Please inquire or refer to our latest catalog)
• Available formats: 48 tests and 96 tests
• Shipping: Free express delivery on orders of 3 or more ELISA kits (any targets)
• Bulk and custom options: Contact our team for large-volume quotes or tailored solutions

Let your next discovery be built on data you can defend. Order the Rat Interleukin 22 (IL-22) ELISA Kit from Yanda Bio today, and expand your inflammatory factor toolbox with a partner who knows detection.