Why Detect Human Apolipoprotein E?
Apolipoprotein E (ApoE) is a liver-synthesized glycoprotein central to lipid metabolism. It mediates the clearance of triglyceride-rich lipoproteins by binding to the LDL receptor and LRP1, making it a key player in cholesterol homeostasis and cardiovascular health. The human APOE gene exists as three major isoforms—ε2, ε3, and ε4—that differentially influence disease risk. The ε4 allele is the strongest known genetic risk factor for late-onset Alzheimer’s disease, while the ε2 allele predisposes carriers to type III hyperlipoproteinemia.
While genotyping identifies APOE alleles, quantifying ApoE protein levels with this human ApoE ELISA kit adds a functional dimension to your research. Whether you are exploring neuroinflammation, atherosclerosis, metabolic syndrome, or therapeutic interventions, accurate ApoE measurement reveals expression patterns and turnover dynamics that genotype alone cannot provide.
Kit Components (96‑Well Configuration)
| Component | Quantity |
|---|---|
| High‑Binding Microplate (Pre‑coated) | 8 wells × 12 strips |
| Standards | 0.3 mL × 6 tubes |
| Sample Diluent | 6 mL |
| HRP‑Conjugated Detection Antibody | 6 mL |
| 20× Wash Buffer | 25 mL |
| Substrate A | 6 mL |
| Substrate B | 6 mL |
| Stop Solution | 6 mL |
| Plate Sealer | 2 sheets |
| Manual | 1 copy |
Assay Principle
This human apolipoprotein E ELISA kit is a ready-to-use, double-antibody sandwich enzyme-linked immunosorbent assay. A capture antibody specific for human ApoE is pre-coated onto the microplate. Standards and samples are loaded, followed by an HRP‑conjugated detection antibody. After incubation and thorough washing, TMB substrate is added. The enzyme reaction produces a color change from blue to yellow upon acid stopping, and the absorbance at 450 nm is directly proportional to the ApoE concentration. Concentrations are calculated from a standard curve.
Kit Performance Characteristics
| Parameter | Specification |
|---|---|
| Accuracy | Standard curve correlation coefficient R ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 0.1 mg/dL |
| Detection Range | 0.1–8 mg/dL |
| Specificity | No cross-reactivity with closely related soluble structural analogs |
| Precision | Intra-assay and inter-assay CV < 15% |
| Shelf Life | 6 months when stored at 2–8°C, protected from light and moisture |
| Sample Types Validated | Serum, plasma, cell culture supernatant, tissue homogenate |
Sample Collection & Storage
- Serum: Collect blood in pyrogen-free tubes, allow clotting, centrifuge at 3000 rpm for 10 min, and harvest the supernatant.
- Plasma: Use EDTA, citrate, or heparin anticoagulants. Centrifuge at 3000 rpm for 30 min, then collect the supernatant.
- Cell culture supernatant: Centrifuge at 3000 rpm for 10 min to remove debris.
- Tissue homogenate: Homogenize tissue in appropriate volume of normal saline, centrifuge at 3000 rpm for 10 min, and collect the supernatant.
- Storage: For delayed testing, aliquot samples and store at -20°C. Avoid repeated freeze-thaw cycles. Thaw completely at room temperature and mix gently before use.
Note: During the assay procedure, samples are diluted 5‑fold. Multiply the calculated result by 5 to obtain the actual sample concentration.
Assay Protocol Summary (Full Instructions Included)
- Equilibrate all reagents and the desired number of pre-coated strips to room temperature for 20 minutes. Reseal unused strips and store at 4°C.
- Standards/Blanks: Add 50 µL of each standard or zero standard to the designated wells.
- Samples: Add 10 µL sample + 40 µL sample diluent per well (blank wells receive neither sample nor standard).
- Add 100 µL HRP‑conjugated detection antibody to each well (except the blank). Cover and incubate at 37°C for 60 minutes.
- Aspirate and wash each well five times with 1X wash buffer (dilute the provided 20× concentrate 1:20 with distilled water; dissolve any salt crystals by warming). Soak 1 minute per wash.
- Add 50 µL each of Substrate A and Substrate B, incubate in the dark at 37°C for 15 minutes.
- Add 50 µL Stop Solution. Read absorbance at 450 nm within 15 minutes and construct a standard curve to calculate concentrations.
Detailed, step-by-step instructions and a troubleshooting guide are provided with every kit.
Why Choose Yanda Bio?
- Manufacturer‑direct quality: Every kit is produced, validated, and lot‑controlled in‑house, ensuring consistent lot‑to‑lot reproducibility.
- Extreme target diversity: Over 6,000 ELISA targets covering human, mouse, rat, pig, rabbit, canine, bovine, chicken, duck, sheep, monkey, fish, insect, plant, and microbial species.
- Academic trust: Long‑term collaborations with more than 60 universities and research institutes in China, supporting high‑impact studies.
- Cost‑effective and convenient: Small or large orders welcome. Buy any 3 kits and get free shipping.
- Custom solutions available: Contact us for bulk orders, custom plate formats, or kit development.
Related ELISA Kits
To build a comprehensive lipid metabolism panel, researchers often pair this assay with:
- Human Apolipoprotein A1 (ApoA1) ELISA Kit
- Human Apolipoprotein B (ApoB) ELISA Kit
(Please insert your internal links here.)
For neurodegenerative disease studies, you may also explore our human Aβ and Tau ELISA kits.
Disclaimer: This kit is for research use only and is not intended for diagnostic or therapeutic procedures. Follow the protocol exactly as provided; deviations are the sole responsibility of the experimenter.
Yanda Bio – Empowering your research with precision ELISA tools.
