HMBS Elisa Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 8*12strips | 8*6strips |
| Standard | 0.3mL*6tubes | 0.3mL*6tubes |
| Sample Diluent | 6.0mL | 3.0mL |
| HRP-Conjugate reagent | 6.0mL | 3.0mL |
| 20X Wash solution | 25mL | 15mL |
| Chromogen Solution A | 6.0mL | 3.0mL |
| Chromogen Solution B | 6.0mL | 3.0mL |
| Stop Solution | 6.0mL | 3.0mL |
| Closure plate membrane | 2 | 2 |
| User manual | 1 | 1 |
Note: Standard (S0 → S5) activity was followed by:0,25,50, 100,200,400 U/L.
Why Choose Yanda Bio’s Human HMBS ELISA Test Kit?
High Sensitivity & Accuracy: Detect HMBS levels with precision using advanced sandwich ELISA technology.
Cost-Effective Pricing: Competitive wholesale prices starting under $100 per kit—ideal for bulk orders and routine lab use.
Ready-to-Use Components: Pre-coated plates, standards, controls, and buffers included for streamlined workflow.
Validated Performance: Rigorously tested for reproducibility, low cross-reactivity, and consistent results.
Detection Principle: Sandwich ELISA Method
The Human HMBS ELISA Test Kit uses a two-step sandwich enzyme-linked immunosorbent assay (ELISA).
Coating: Microplate wells are pre-coated with a Human HMBS-specific antibody.
Sample Incubation: Samples or standards are added, and HMBS binds to the immobilized antibody. After incubation and washing, only bound antigens remain.
Detection Antibody Addition: Horseradish peroxidase (HRP)-conjugated detection antibody is added, forming an antibody–HMBS–antibody “sandwich” complex.
Signal Development: TMB substrate is added. HRP catalyzes the conversion of TMB to a blue product, which turns yellow after acid stop solution is applied.
Measurement: Absorbance is measured at 450 nm using a microplate reader. The intensity of yellow color is directly proportional to the concentration of Human HMBS in the sample.
Applications
Research: Study HMBS function in heme biosynthesis, metabolic disorders, and enzyme kinetics.
Diagnostics: Potential tool for porphyria-related studies and metabolic condition screening.
Pharmaceutical Development: Monitor HMBS expression in drug efficacy and toxicology studies.
