Human CCL18 ELISA Kit: For Immune Regulation & Fibrosis Research
Yanda Bio’s Human CCL18 ELISA Kit is designed for the specific and sensitive detection of C-C Motif Chemokine Ligand 18 (also known as PARC or AMAC-1) in human serum, plasma, cell culture supernatants, and tissue lysates. As a chemokine predominantly produced by antigen-presenting cells, CCL18 plays a pivotal role in regulating immune tolerance, T cell recruitment, and is strongly implicated in fibrotic diseases and cancer progression. This kit provides researchers in immunology, rheumatology, oncology, and pulmonology with a reliable tool to quantify this important biomarker.
As a factory-direct source with dedicated R&D facilities in China, we offer premium quality with exceptional value. This ELISA kit made in China is available at a retail price of $120, with bulk pricing under $100. Our capabilities are extensive, featuring a portfolio of over 6,000 detection targets across immunology, oncology, neuroscience, and more, supported by collaborations with 70+ universities.
Two Optimized Tiers to Match Your Research Needs
To address varying sensitivity requirements and budgets, we offer two distinct kit configurations. Purchasing either tier includes our complimentary expert sample testing service.
| Feature | Tier 1: Standard Sensitivity CCL18 ELISA Kit | Tier 2: High-Sensitivity CCL18 ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with a proprietary matrix-suppression diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) paired with Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Benefit | Excellent Value & Robustness. Employs a single-step incubation and a specialized diluent that effectively reduces background noise from hemoglobin and lipids, ensuring consistent performance in complex samples. | Superior Sensitivity & Specificity. The advanced biotin-streptavidin amplification directly counteracts matrix interference, providing an outstanding signal-to-noise ratio for detecting low-abundance CCL18 or analyzing challenging sample types. |
| Best Suited For | Routine monitoring and studies where CCL18 concentrations are expected to be within a standard detectable range. | High-stakes research requiring the lowest possible detection limits, analysis of limited sample volumes, or work with highly complex biological matrices. |
Complimentary Expert Testing Service: Data You Can Trust, Delivered Fast
Save valuable laboratory time and leverage our expertise. Our professional service is included with your purchase.
- Guaranteed Fast Start: We initiate your assay within 24 hours of sample arrival.
- Rapid, Comprehensive Reporting: Receive a complete data package in approximately 5 business days, including raw OD values, calculated concentration tables, and a standard curve graph.
- Seamless Validation: An ideal solution for verifying assay performance with your specific samples prior to establishing an in-house protocol.
Our Commitment: Built on Trust and Scientific Integrity
We believe in supporting your research with unwavering commitments:
- Publication Acknowledgement: Cite this Human CCL18 ELISA Kit and link to our product page in your publication, and we will reward your contribution with a free standard ELISA kit (up to $120 value).
- 100% Satisfaction Guarantee: We promise performance. If any product fails to meet its published specifications, we offer an unconditional replacement or refund.
- Responsive Support Pledge: Our dedicated team provides a clear path to resolution within 24 hours of any query.
- Guardians of Your Research: We treat every sample with the utmost care, applying rigorous standards to ensure the integrity of your scientific work.
Technical Specifications & Assay Principle
Detection Principle: The kit utilizes a validated one-step sandwich ELISA. CCL18 present in the sample is captured between an immobilized antibody and an HRP-conjugated detection antibody during a single incubation. The subsequent enzymatic reaction with TMB substrate produces a colorimetric signal measured at 450 nm, which is directly proportional to the concentration of CCL18.
Performance Characteristics:
- Detection Range: Typically 10 – 800 pg/mL (exact range per batch).
- Sensitivity: Typically < 10 pg/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- and Inter-assay Coefficient of Variation (CV) < 15%.
- Specificity: High specificity for human CCL18, with minimal cross-reactivity.
- Storage & Stability: Store at 2-8°C; stable for 6 months.
Sample Handling: For optimal results, clarify serum, plasma, or cell culture media by centrifugation (e.g., 3000×g for 10 minutes). Aliquot and store samples at -20°C, avoiding repeated freeze-thaw cycles.
Streamlined Workflow Overview
- Addition: Pipette 50 µL of standards or prepared samples into the pre-coated wells.
- Incubation: Add 100 µL of the HRP-conjugated Detection Antibody. Incubate at 37°C for 60 minutes.
- Wash: Aspirate and wash the microplate 5 times thoroughly with the provided wash buffer.
- Development: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes, protected from light.
- Measurement: Add 50 µL Stop Solution. Measure the absorbance at 450 nm within 15 minutes.
Ordering Information & Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Cost-effective, reliable solution for in-laboratory use. | Retail: $120. Bulk: < $100 (quote required). |
| Tier 2: High-Sensitivity Kit | Premium performance for maximum sensitivity requirements. | Contact for competitive manufacturer price. |
| Expert Testing Service | Full-service assay execution and detailed data reporting. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored assay development from our vast portfolio of 6,000+ targets. | Project-based quotation available. |
To order your Human CCL18 ELISA Kit or to explore our extensive catalog, contact Yanda Bio, your trusted direct manufacturer.
Research Applications & Frequently Asked Questions
Primary Applications (Research Use Only): Studies in idiopathic pulmonary fibrosis (IPF), liver fibrosis, rheumatoid arthritis, cancer immunology (particularly breast and ovarian cancer), allergic inflammation, and general immunoregulation.
Q: What is the biological significance of measuring CCL18?
A: CCL18 is a key marker for alternatively activated macrophages and is strongly associated with fibrotic disease activity and poor prognosis in certain cancers, making it a valuable biomarker for mechanistic and clinical research.
Q: How do I claim the free kit for a publication?
A: Once your peer-reviewed article citing the “Human CCL18 ELISA Kit (Yanda Bio)” with a direct link to our product page is published, send us the citation. We will promptly fulfill your complimentary kit reward.
Q: How can I access your portfolio of 6,000+ assays?
A: Please contact our technical support team with your target of interest. We can quickly provide availability, specifications, or discuss custom development possibilities.
Q: Why choose a direct manufacturer in China?
A: Choosing Yanda Bio means eliminating intermediaries, ensuring competitive pricing (manufacturer price), receiving direct technical support, and benefiting from in-house quality control throughout the production process.
Manufacturer’s Note
This product is developed and sold for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical procedures. The researcher is responsible for determining the suitability of this kit for their specific application.
