The Rat Neurofilament Protein Peptide (NFL) ELISA Kit is a highly sensitive and specific assay for the quantitative detection of neurofilament light polypeptide (NFL), a major structural component of the neuronal cytoskeleton. NFL resides primarily within neurons with minimal release into extracellular fluids; however, axonal injury, demyelination, or neurodegeneration triggers substantial NFL efflux into cerebrospinal fluid (CSF) and peripheral blood, establishing NFL as a well‑validated biomarker of neuronal damage. This rat NFL ELISA kit is widely used in neurodegenerative disease research, including amyotrophic lateral sclerosis (ALS) and Huntington’s disease, to track disease progression and evaluate therapeutic interventions. In acute brain injury models, such as stroke and traumatic brain injury, the assay enables dynamic monitoring of irreversible axonal damage. For neuroimmune and demyelinating disorders, it serves as a quantitative tool to assess disease activity and treatment response in conditions like neuromyelitis optica spectrum disorder (NMOSD). Additionally, neuropharmacology researchers rely on this kit to evaluate the neuroprotective effects or potential neurotoxicity of drug candidates in preclinical rat studies.

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As a leading ELISA kit manufacturer in China, Yanda Bio provides premium-quality, affordable ELISA kits directly from our state-of-the-art R&D and production facilities. Buy the Rat Neurofilament Protein Peptide (NFL) ELISA Kit with the authentic manufacturer price, ensuring you receive the best value without intermediaries.

Rat NFL ELISA Kit Materials supplied

Note: Standard (S0 → S5) concentration was followed by:0,7.5, 15,30,60, 120 pg/ mL

Reagent preparation

20×wash solution:Dilute with Distilled or deionized water 1:20.

Why Choose Yanda Bio’s Rat NFL ELISA Kit?

1. Unbeatable Price Advantage: We are the source factory. Our standard human ELISA kits in retail-priced. Enjoy genuine cost savings without compromising quality.
2. Professional Testing Service Included: Purchase any Yanda Bio ELISA kit and benefit from our complimentary professional assay service. Simply send your samples. We initiate experiments within 24 hours of sample receipt and deliver results in as fast as 5 working days.
3. Comprehensive Data Delivery: Receive a complete data package including raw data, concentration calculation tables, and standard curve graphs for transparent and publication-ready results.
4. Dual-Format Kit Options for Flexible Needs:
   • Standard Format: Features a one-step co-incubation process where the antigen simultaneously binds to the solid-phase antibody and enzyme-labeled antibody. The unique dilution buffer effectively reduces interference from substances like hemoglobin and lipids, minimizing background noise and ensuring optimal binding.
   • High-Sensitivity Format: Utilizes high-purity (>95%) biotinylated recombinant monoclonal antibodies paired with Streptavidin-HRP conjugates (activity ≥500 U/mg) for significant signal amplification. This format directly minimizes matrix effects and maximizes specificity for challenging samples.
5. Trusted by Academia: We are proud to collaborate with over 70 universities worldwide, who rely on our reagents for their critical research.

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Our Service Commitment:

• Custom ELISA Kit Development: We offer tailored ELISA kit development services to meet your specific research requirements.
• Citation Incentive: Publish your research citing the use of our Rat NFL ELISA Kit and linking to our website, and we will reward you with a free standard ELISA kit of your choice.
• 100% Satisfaction Guarantee: We stand firmly behind our products. If the kit does not perform as specified, we offer a no-questions-asked replacement or refund. Our dedicated support team provides a concrete solution within 24 hours of any inquiry.
• Your Research, Our Responsibility: We understand the irreplaceable value of every sample and the importance of each experiment. Our team is committed to supporting your scientific journey with professional expertise, rigorous quality control, and sincere partnership.

Order Your Rat NFL ELISA Kit Today from Yanda Bio – Where Premium Quality Meets Direct Manufacturer Value.Blazing hot price: 120 $

Calculation of results

1.    This  standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.

2.    First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.

3.    To  determine  the  amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.

4.    Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit

age can cause variation in result. Each user should obtain their own standard curve.

5.    The sensitivity by this assay is 0. 1pg/ mL

6.    Standard curve