Rat OXCT1 ELISA Kit – Quantitative Detection of the Key Ketolytic Enzyme

The Rat 3-Oxoacid CoA-Transferase 1 (OXCT1) ELISA Kit provides accurate quantitative measurement of OXCT1, the rate‑limiting enzyme that catalyzes ketone body catabolism (ketolysis) in extrahepatic tissues, making it an indispensable tool for cellular energy metabolism studies. This rat OXCT1 ELISA kit enables metabolic research by assessing ketone body utilization levels under diverse physiological and pathological conditions in rodent models. In cancer research, OXCT1 is commonly overexpressed across multiple tumor types, with expression levels positively correlating with malignancy and aggressive tumor behavior. Consequently, the kit is widely applied to investigate cancer metabolic reprogramming, allowing researchers to explore the mechanisms that fuel tumor cell proliferation and survival, and to evaluate OXCT1 as a potential metabolic vulnerability or therapeutic target.

Rat OXCT1 ELISA Kit Supplied

Note: Standard (S0 → S5) concentration was followed by:0 、2.5 、5 、10 、20 、40U/ L

Target Introduction: OXCT1 in Metabolic Research

3-Oxoacid CoA-Transferase 1 (OXCT1), also known as Succinyl-CoA:3-ketoacid coenzyme A transferase, is a critical mitochondrial enzyme essential for ketone body utilization. It catalyzes the first step in converting acetoacetate into acetoacetyl-CoA, making it a key player in energy metabolism, especially in the heart, brain, and kidneys during periods of fasting or high-fat diets. Accurate measurement of Rat OXCT1 activity is vital for studies in metabolic disorders, diabetes, cardiac energetics, and neurobiology.

Yanda Bio’s Rat OXCT1 ELISA Kit offers a precise and convenient tool for researchers in these fields. As a specialized manufacturer with expertise across 6,000+ detectable targets and trusted through long-term collaborations with over 60 universities, we provide reliable, cost-effective rat ELISA Kit solutions to support high-impact science.

Unmatched Value: Premium Quality Meets Efficient Production

Investing in reliable reagents shouldn’t strain your budget. The core of assay performance lies in antibody specificity and the stability of enzyme conjugates—components where premium brands traditionally invest.

Our Commitment to Quality: We source high-specificity antibodies and high-activity enzyme conjugates comparable to those used in leading international kits. Our optimized ELISA Kit production and direct-to-researcher model eliminate unnecessary markups, delivering this premium performance within an accessible, low-cost ELISA Kit framework. Contact us and we will tell you the genuine price of a high-quality Elisa kit..

Two Optimized Formats: Precision for Every Protocol

We offer this assay in two specialized configurations to match your experimental priorities—whether maximizing budget efficiency or ensuring utmost sensitivity for critical samples.

Format A: High-Efficiency Standard Kit

Ideal for routine assessment of OXCT1 activity where sample volume is sufficient. This kit utilizes a streamlined one-step incubation process and a proprietary matrix-optimized diluent. The diluent effectively minimizes interference from hemoglobin, lipids, and other common sample components, ensuring stable baselines and robust, reproducible results (validated success rate >99%). It is our recommended solution for cost-effective routine analysis.

• Typical Pricing: Around $120 (retail). Under $100 per unit for wholesale or institutional purchases.

Format B: Advanced Sensitivity & Specificity Kit

Designed for challenging studies involving low-activity samples, precious biopsies, or complex matrices. This format utilizes a biotinylated recombinant monoclonal antibody (purity >95%) combined with a high-activity Streptavidin-HRP complex (≥500 U/mg). This system provides powerful signal amplification, effectively circumvents matrix effects, and is engineered for success in experiments where material is limited or failure is not an option.

Guidance for Selection:

• Choose Format A for reliable, budget-conscious measurement in standard metabolic phenotyping studies.
• Choose Format B for projects involving trace-level activity detection, irreplaceable samples, or when working with challenging sample types that require maximum assay robustness.

Assay Principle & Key Features

This kit is based on a sandwich ELISA principle designed to measure OXCT1 protein levels as a correlate of activity. The plate is pre-coated with a capture antibody specific to Rat OXCT1. After binding of the target in samples and standards, an HRP-conjugated detection antibody completes the “sandwich.” The enzymatic reaction with TMB substrate yields a colorimetric signal proportional to the amount of OXCT1 present.

Important Experimental Note: For optimal results, we recommend performing a pilot experiment to determine the optimal sample dilution. This step helps prevent a potential “hook effect” (prozone effect) in one-step sandwich ELISA designs, where extremely high analyte concentrations can lead to falsely low signals, thereby ensuring the accuracy of your final data.

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Why Partner with Yanda Bio?

• Direct Value: Access premium-grade assay components through our efficient, cost-effective rat ELISA Kit supply chain.
• Free Assay Service: Purchase any Yanda Bio ELISA kit and receive complimentary expert testing—we generate the data from your provided samples.
• Publication Support: Cite our kit and link to our product page in your research article to receive a free standard ELISA kit.
• 100% Quality Guarantee: We confidently back our products with an unconditional promise: if performance deviates from specifications, you receive a full replacement or refund.
• Expert Technical Support: Connect with our specialists and receive a targeted troubleshooting or consultation response within 24 hours.
• Custom Assay Development: Explore bespoke solutions with our end-to-end custom ELISA kit services.

Specifications at a Glance

• Accuracy: Standard curve linear regression correlation coefficient (R) ≥ 0.9900.
• Precision: Intra-assay Coefficient of Variation (CV) < 10%; Inter-assay CV < 15%.
• Specificity: No significant cross-reactivity with other relevant soluble proteins.
• Sample Types: Validated for serum, plasma (EDTA, citrate, heparin), cell culture supernatant, and tissue homogenate.
• Kit Stability: Stable for 6 months when stored at 2-8°C.