Product Overview: Understanding 2,3-Diphosphoglycerate (2,3-DPG)
2,3-Diphosphoglycerate (2,3-DPG) is a crucial intermediate metabolite in the glycolytic pathway of red blood cells (Rapoport-Luebering shunt). Its primary function is to regulate the oxygen affinity of hemoglobin; increased 2,3-DPG levels decrease hemoglobin’s oxygen-binding affinity, facilitating oxygen release to tissues. Accurate quantification of Rat 2,3-DPG is therefore essential for research in high-altitude physiology, anemia, blood storage (banking), metabolic disorders, and conditions involving tissue hypoxia or altered oxygen delivery.
Yanda Bio’s Rat 2,3-Diphosphoglycerate (2,3-DPG) ELISA Kit provides researchers with a robust and reliable tool for precise measurement of this vital metabolite. As an established leader in ELISA Kit production with expertise across 6,000+ detectable targets and strong collaborative ties to over 60 domestic universities, we are committed to delivering high-performance, cost-effective rat ELISA Kit solutions that meet the rigorous demands of modern life science research.
Premium Quality for Your Research, Priced for Accessibility
A reliable ELISA requires high-specificity antibodies and a robust, reproducible protocol—attributes that often define expensive commercial kits. We believe professional-grade tools should be within every researcher’s reach.
Our Value Promise: We utilize high-specificity antibodies and premium-grade assay components that meet stringent performance benchmarks, comparable to those used by leading international suppliers. Our streamlined, efficient ELISA Kit production process and direct-to-researcher model allow us to deliver this quality within a truly affordable rat ELISA Kit framework. This makes our kits a genuinely cheap ELISA Kit alternative, offering substantial savings over premium brands without compromising on the sensitivity or reproducibility required for critical metabolic studies.
Our Expertise: While we primarily specialize in high-quality sandwich ELISA formats as featured here, our capabilities extend to other assay methodologies, including competitive ELISA formats. Please inquire if your specific target or application requires an alternative testing approach.
Two Optimized Formats: Designed for Your Specific Research Needs
To provide the best fit for every experimental design and budget, we offer this assay in two performance-calibrated formats.
Format 1: High-Efficiency Standard Kit
Ideal for routine, high-confidence quantification of 2,3-DPG in standard sample types. This kit features a simplified one-step incubation protocol, reducing total hands-on time. It includes a proprietary matrix-optimization diluent engineered to neutralize interference from hemoglobin (particularly relevant for erythrocyte lysates) and lipids, ensuring a stable, low-background signal and excellent inter-assay consistency. With extensive validation demonstrating a success rate exceeding 99%, this is our most accessible and low-cost ELISA Kit solution.
- Typical Pricing: Approximately $120 (retail). Under $100 per kit for wholesale, bulk, or institutional purchase plans.
Format 2: Advanced High-Sensitivity Kit
Developed for the most demanding applications where sample volume is extremely limited (e.g., neonatal rat studies, serial sampling) or where metabolite concentrations are expected to be exceptionally low. This advanced system employs a biotinylated recombinant monoclonal antibody (purity >95%) in combination with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This sophisticated signal amplification technology delivers maximum sensitivity, directly overcomes challenging matrix effects, and is engineered to guarantee success in pivotal experiments with irreplaceable samples.
Selection Guide:
- Choose Format 1 for robust, economical, and reliable analysis in standard research settings with sufficient sample material.
- Choose Format 2 when your project demands the highest detection sensitivity for trace-level analysis, involves extremely scarce samples, or requires maximum robustness against complex sample backgrounds.
Assay Principle: Reliable Sandwich ELISA Methodology
This kit is based on a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) design. A capture antibody specific for Rat 2,3-DPG is immobilized on the microplate. During incubation, the 2,3-DPG present in standards and prepared samples binds to these coated antibodies. Following a wash step, a Horseradish Peroxidase (HRP)-conjugated detection antibody is added, forming an antibody-antigen-antibody “sandwich” complex. After a subsequent wash to remove unbound material, a TMB substrate solution is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which turns yellow upon acidification. The optical density (OD) of this yellow color, measured at 450 nm, is directly proportional to the concentration of 2,3-DPG in the original sample.
Why Partner with Yanda Bio?
- Unbeatable Value: Obtain premium assay performance through our optimized, cost-effective rat ELISA Kit supply chain, offering significant cost advantages versus major international suppliers.
- Complimentary Expert Analysis Service: Purchase any Yanda Bio ELISA kit and receive FREE expert-assisted testing. Provide your prepared samples, and our technical team will generate and deliver the validated data.
- Publication Support Program: Acknowledge the use of our product with a citation and direct link in your peer-reviewed article to receive a free standard ELISA kit as our contribution to your research success.
- Unconditional Quality Guarantee: We stand firmly behind our承诺. If the product’s performance does not meet the published specifications, we offer a no-questions-asked replacement or full refund.
- Rapid & Responsive Support: Our dedicated technical support team is committed to providing a detailed, actionable response to any inquiry within 24 hours.
- Custom Assay Development: For research on targets not yet in our expansive catalog of 6,000+ factors, we offer end-to-end custom ELISA kit services tailored to your unique requirements.
Detailed Performance Specifications
- Accuracy & Linearity: The standard curve demonstrates a linear regression correlation coefficient (R) of ≥ 0.9900.
- Precision: Both intra-assay (within-run) and inter-assay (run-to-run) Coefficients of Variation (CV) are consistently less than 15%.
- Specificity: The assay shows no significant cross-reactivity with other phosphorylated glycolytic intermediates or structurally similar small molecules.
- Sample Compatibility: Validated for use with rat hemolyzed erythrocyte preparations, serum, and plasma (EDTA, citrate, heparin).
- Kit Stability & Shelf Life: The complete kit is stable for 6 months from the date of manufacture when stored continuously at 2-8°C.
Ordering & Contact Details
To purchase this Rat 2,3-Diphosphoglycerate (2,3-DPG) ELISA Kit for sale, or to discuss volume discounts, specific project requirements, and our custom assay services, please contact our sales team using the product code provided.
