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Rat Monoacylglycerol (MAG) ELISA Kit | Precise Lipid Metabolite Quantification

Product Code: YD-R-MAG
Application: For the specific and quantitative measurement of Rat Monoacylglycerol (MAG, such as 2-Monoacylglycerol) in serum, plasma, tissue homogenates, and cell lysates.
Detection Range: 0.1 – 80 U/mL
Sensitivity: < 0.1 U/mL Sample Volume: 10 μL Total Assay Time: ~1 hour 45 minutes Storage: 2-8°C, protect from light Format: 96-well pre-coated plate [Buy Rat Diacylglycerol (MAG) ELISA Kit Online] | [Request a Bulk Quote & Custom Service Inquiry]

Rat Monoacylglycerol (MAG) ELISA Kit supplied

Name96 determinations48 determinations
Microelisa stripplate12*8strips12*4strips
Standard0.3ml*6tubes0.3ml*6tubes
Sample Diluent6.0ml3.0ml
HRP-Conjugate reagent10.0ml5.0ml
20X Wash solution25ml15ml
Chromogen Solution A6.0ml3.0ml
Chromogen Solution B6.0ml3.0ml
Stop Solution6.0ml3.0ml
Closure platemembrane22
User manual11
Sealed bags11

Note: Standard (S0 → S5) concentration was followed by:0, 1.25,2.5,5, 10,20 ng/mL


Product Introduction: The Role of Monoacylglycerol (MAG) in Lipid Metabolism

Rat Diacylglycerol (MAG) ELISA Kit for sale. Monoacylglycerol (MAG) is a pivotal intermediate in both the digestion and intracellular metabolism of lipids. Primarily, 2-Monoacylglycerol is a key product of dietary fat breakdown and serves as a critical substrate for lipid resynthesis. Beyond its metabolic role, MAG species also function as important lipid signaling molecules. Accurate quantification of Rat Monoacylglycerol (MAG) is therefore crucial for research in energy metabolism, obesity, diabetes, gastrointestinal physiology, and lipid-mediated signal transduction pathways.

Yanda Bio’s Rat Monoacylglycerol (MAG) ELISA Kit is engineered to deliver sensitive and reliable data for this essential lipid metabolite. As a dedicated manufacturer in the field of ELISA Kit production, with a vast portfolio of over 6,000 detectable factors and enduring collaborations with more than 60 Chinese universities, we provide rigorously validated, highly cost-effective rat ELISA Kit solutions for complex lipid research.


Premium Assay Quality at a Truly Accessible Price

The accuracy of lipid metabolite measurement depends heavily on the specificity of the immunoassay’s antibodies. Premium international kits set a high benchmark but often at a prohibitive cost for routine or high-throughput studies.

Our Value Commitment: We utilize highly specific antibodies against key MAG species, ensuring analytical precision comparable to leading brands. By optimizing our in-house ELISA Kit production and maintaining a direct-to-researcher supply model, we integrate this quality into a remarkably affordable rat ELISA Kit. This approach makes our kits a genuinely cheap ELISA Kit alternative, offering researchers a low price without the typical compromise in performance required for demanding lipidomics and metabolic studies.

Assay Format Expertise: While this specific kit utilizes a sensitive sandwich ELISA format, our technical capabilities extend to other methodologies, including competitive ELISA formats. Please contact us to discuss the optimal assay design for your unique research target.


Dual-Format Design: Optimized for Every Research Goal and Budget

To support diverse experimental needs—from high-volume screening to the analysis of precious, limited samples—we offer this kit in two performance-calibrated formats.

Format 1: Standard High-Value Kit
Designed for robust, routine quantification of MAG in standard sample matrices. This format employs an efficient one-step incubation, minimizing hands-on time. It features a proprietary diluent engineered to neutralize interference from high levels of lipids, hemoglobin, and other serum components, ensuring a clean background and reproducible results. With extensive validation across numerous applications, this format boasts a demonstrated success rate greater than 99% and represents our most low-cost ELISA Kit option.

  • Typical Pricing: Approximately $120 (retail). Bulk pricing starts under $100 per kit, offering significant savings for laboratories.

Format 2: Advanced High-Sensitivity Kit
Developed for challenging research where metabolite concentrations are very low or sample material is scarce and irreplaceable (e.g., specific tissue biopsies, micro-samples). This advanced system leverages a biotinylated recombinant monoclonal antibody (purity >95%) combined with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This powerful signal amplification technology maximizes sensitivity, directly overcomes complex matrix effects, and is engineered to guarantee success in critical, one-attempt experiments.

Selection Guide for Optimal Value:

  • Choose Format 1 for reliable and economical analysis in studies with ample sample volume.
  • Choose Format 2 when your research requires the ultimate detection limit, involves trace-level analysis of rare samples, or demands maximum assay robustness against challenging biological matrices.

Assay Principle: Reliable Sandwich ELISA Methodology

This kit operates on a well-established sandwich enzyme-linked immunosorbent assay (ELISA) principle. A capture antibody specific for Rat MAG is immobilized on the microplate. During incubation, the MAG present in standards and prepared samples binds to these antibodies. After washing, a Horseradish Peroxidase (HRP)-conjugated detection antibody is added, forming an antibody-antigen-antibody “sandwich” complex. Following another wash step, a TMB substrate solution is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which turns yellow upon acidification. The intensity of this yellow color, measured at 450 nm, is directly proportional to the concentration of MAG in the original sample.


Comprehensive Support for Your Research Success

  • Exceptional Value: Access premium immunoassay performance through our streamlined, cost-effective rat ELISA Kit model, delivering substantial savings versus major international suppliers.
  • Free Expert-Assisted Testing: Purchase any Yanda Bio ELISA kit and qualify for FREE data generation service. Provide your samples, and our specialists will perform the assay and deliver validated results.
  • Publication Incentive Program: Acknowledge the use of our kit with a citation and direct link in your peer-reviewed manuscript to receive a complimentary standard ELISA kit.
  • Unconditional Quality Guarantee: We confidently stand behind our products. If performance does not meet published specifications, we offer a prompt replacement or full refund.
  • 24-Hour Technical Support: Our dedicated team is committed to providing an expert, actionable response to any technical inquiry within one business day.
  • Custom Assay Development: For novel or specialized targets, we offer comprehensive custom ELISA kit design and production services tailored to your specific research objectives.

Detailed Performance Specifications

  • Accuracy & Linearity: The standard curve exhibits a linear regression correlation coefficient (R) of ≥ 0.9900.
  • Precision: Intra-assay and inter-assay Coefficients of Variation (CV) are consistently less than 15%.
  • Specificity: The assay demonstrates negligible cross-reactivity with other related glycerolipids (e.g., diacylglycerols, triacylglycerols) or structural analogs.
  • Sample Compatibility: Validated for use with rat serum, plasma (EDTA, citrate, heparin), tissue homogenates, and cell lysates.
  • Kit Stability & Shelf Life: The complete kit is stable for 6 months from the date of manufacture when stored continuously at 2-8°C.

Ordering & Contact Information

To order this Rat Diacylglycerol (MAG) ELISA Kit, or to discuss volume pricing, specific project consultations, and custom assay services, please contact our sales team using the product code provided.

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