Product Overview: Understanding Type I Collagen in Musculoskeletal & Connective Tissue Research
Type I Collagen (Col‑I) is the most abundant collagen in the human and rabbit body, accounting for approximately 90 % of the total collagen mass. It is the primary structural component of bone, tendon, ligament, skin, cornea, and dentin. Col‑I provides tensile strength, tissue integrity, and serves as a scaffold for cell adhesion and mineralization. Accurate quantification of Rabbit Type I Collagen (Col‑I) is essential for research in bone metabolism, osteoporosis, fracture healing, tendon/ligament repair, dermal wound healing, fibrosis, and connective tissue disorders. Rabbit models are widely used in orthopaedic and dermatological studies, making a reliable Col‑I assay indispensable.
Yanda Bio’s Rabbit Type I Collagen (Col‑I) ELISA Kit employs a sensitive sandwich immunoassay format to specifically detect native and denatured Col‑I fragments. As a professional ELISA kit manufacturer with a portfolio exceeding 6,000 detectable targets and long‑term collaborations with over 60 universities across China, we bring deep expertise in extracellular matrix biology to support your musculoskeletal and connective tissue research.
Explore Our Complete Rabbit Collagen Series:
- Rabbit Type III Collagen (Col‑III) ELISA Kit — For fibrosis, wound healing, and vascular matrix studies
- Rabbit Type II Collagen (Col‑II) ELISA Kit — For cartilage, osteoarthritis, and joint research
Why Choose Yanda Bio for Your Collagen Immunoassays?
EEAT‑Compliant Quality Standards
Our commitment to Experience, Expertise, Authoritativeness, and Trustworthiness ensures that every kit meets the highest standards for extracellular matrix protein quantification.
| Principle | Our Commitment |
|---|---|
| Experience | Over 6,000 detectable factors developed; 60+ university collaborations; years of dedicated work in collagen immunoassay development. |
| Expertise | Proprietary antibody pairs validated for rabbit‑specific collagen subtypes; sandwich ELISA design ensures minimal cross‑reactivity between Col‑I, Col‑II, and Col‑III. |
| Authoritativeness | Kits are validated in academic research settings; ISO‑aligned manufacturing; rigorous lot‑to‑lot quality control. |
| Trustworthiness | Rapid custom development (3–7 days); transparent specifications; unconditional quality guarantee. |
Superior Quality Metrics
- Intra‑assay CV < 5 % — Exceptional within‑run precision
- Inter‑assay CV < 8 % — Outstanding run‑to‑run consistency
- High Specificity — No significant cross‑reactivity with rabbit Col‑II, Col‑III, or other collagen types
Kit Components: What’s Included
Our Rabbit Col‑I ELISA Kit is available in two convenient configurations.
| Component | 96‑Well Configuration | 48‑Well Configuration |
|---|---|---|
| Microplate (Pre‑coated) | 8 wells × 12 strips | 8 wells × 6 strips |
| Standards | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes |
| Sample Diluent | 6 mL | 3 mL |
| HRP‑Conjugated Detection Antibody | 6 mL | 3 mL |
| 20× Wash Buffer | 25 mL | 15 mL |
| Substrate A | 6 mL | 3 mL |
| Substrate B | 6 mL | 3 mL |
| Stop Solution | 6 mL | 3 mL |
| Plate Sealer | 2 sheets | 2 sheets |
| Manual | 1 copy | 1 copy |
Assay Principle: Sandwich ELISA Technology
This kit uses a solid‑phase sandwich enzyme‑linked immunosorbent assay. The microplate is pre‑coated with a monoclonal antibody specific for Rabbit Type I Collagen (Col‑I). Standards and samples are added, allowing Col‑I to bind to the immobilized antibody. After washing, a Horseradish Peroxidase (HRP)‑conjugated detection antibody is added, forming an antibody‑antigen‑antibody “sandwich”. Following a second wash, TMB substrate is added. The HRP enzyme catalyses a blue colour that turns yellow upon acidification. The optical density (OD) at 450 nm is directly proportional to the concentration of Col‑I in the sample.
Sample Dilution Note: Samples are pre‑diluted 5‑fold during the procedure; multiply results by 5 to obtain actual concentrations.
Sample Collection & Preparation
Correct handling is critical for reproducible collagen quantification.
| Sample Type | Collection & Processing |
|---|---|
| Serum | Use pyrogen/endotoxin‑free tubes. Avoid cell stimulation. Centrifuge at 3000 rpm for 10 minutes. |
| Plasma | Use EDTA, citrate or heparin anticoagulant. Centrifuge at 3000 rpm for 30 minutes. |
| Cell Supernatant | Centrifuge at 3000 rpm for 10 minutes to remove particles. |
| Tissue Homogenate (bone, tendon, skin) | Mince tissue, add PBS (1:9 w/v), homogenise on ice, centrifuge at 5000×g for 10 minutes at 2–8 °C. Collect supernatant. |
| Storage | Aliquot and store at –20 °C (≤ 1 month) or –80 °C (≤ 2 months). Avoid repeated freeze‑thaw cycles. |
Materials Required But Not Provided
- Microplate reader (450 nm)
- Precision pipettes and tips (0.5–10 μL, 2–20 μL, 20–200 μL, 200–1000 μL)
- 37 °C incubator or water bath
Important Notes for Assay Procedure
- Store the kit at 2–8 °C. Bring all reagents to room temperature for 20 minutes before use. Concentrated wash buffer may crystallise; dissolve crystals in a warm water bath.
- Return unused strips to the resealable bag with desiccant and store at 2–8 °C. Use promptly.
- The S0 standard (zero concentration) is the negative control/blank. Samples are diluted 5‑fold; multiply results by 5.
- Strictly adhere to incubation times, volumes and sequence.
- Mix all liquid components thoroughly before use.
Reagent Preparation
20× Wash Buffer Dilution: Dilute 1 part 20× wash buffer with 19 parts distilled water to obtain 1× working wash buffer.
Washing Method
Manual: Aspirate liquid, fill each well with wash buffer, stand for 1 min, aspirate, and tap dry on absorbent paper. Repeat 5 times.
Automatic washer: Inject 350 μL per well, soak for 1 min, wash 5 times.
Assay Procedure
- Remove required strips after 20 min equilibration. Reseal unused strips and return to 4 °C.
- Standard wells: add 50 μL of each standard concentration.
- Sample wells: add 10 μL sample + 40 μL sample diluent. Blank wells: leave empty.
- Add 100 μL HRP‑conjugated detection antibody to all wells except blanks. Seal and incubate at 37 °C for 60 min.
- Aspirate, wash 5 times (manual or automatic).
- Add 50 μL Substrate A and 50 μL Substrate B to each well. Incubate at 37 °C in the dark for 15 min.
- Add 50 μL stop solution. Measure OD at 450 nm within 15 min.
Data Analysis
Plot standard curve: OD values (y‑axis) vs. standard concentrations (x‑axis) in Excel or curve‑fitting software (linear regression, R ≥ 0.9900). Calculate sample concentrations from the curve, then multiply by the dilution factor (5×).
Kit Performance Specifications
| Parameter | Performance |
|---|---|
| Accuracy | Standard curve linear regression coefficient (R) ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 1.0 pg/mL |
| Detection Range | 1.0 – 40 pg/mL |
| Specificity | No cross‑reactivity with rabbit Col‑II, Col‑III, or other soluble collagens |
| Intra‑assay CV | < 5 % |
| Inter‑assay CV | < 8 % |
| Storage | 2–8 °C, protect from light and moisture |
| Shelf Life | 6 months |
Customization Options
Yanda Bio offers custom ELISA kit development services with a rapid 3–7 day turnaround. We can tailor assays for:
- Other species (mouse, rat, human, horse, etc.)
- Different sample matrices (bone extracts, culture media, synovial fluid)
- Adjusted detection ranges and sensitivity
- High‑sensitivity biotin‑streptavidin format
Contact us to discuss your specific needs.
Related Products
Complete Your Rabbit Collagen Panel:
- Rabbit Type III Collagen (Col‑III) ELISA Kit — For fibrosis, vascular and wound healing studies
- Rabbit Type II Collagen (Col‑II) ELISA Kit — For cartilage and osteoarthritis research
Using all three kits together allows you to monitor differential collagen remodeling in bone, cartilage, tendon, skin, and fibrotic tissues.
Special Offer
Buy any three ELISA kits from Yanda Bio and enjoy free shipping. Combine Rabbit Col‑I, Col‑II and Col‑III kits to build a comprehensive extracellular matrix research panel at exceptional value.
Comparison with Premium International Brands
Yanda Bio’s ELISA kits deliver performance equivalent to leading international brands at 40–60 % lower cost. Our direct‑to‑researcher model and efficient manufacturing eliminate unnecessary markups. With superior precision (CV < 5 % intra‑assay, < 8 % inter‑assay) and 3–7 day custom development, we offer an unbeatable combination of quality, speed and affordability.
Disclaimer
Strict adherence to the protocol is required. Yanda Bio is not responsible for results obtained from deviations. This product is for research use only; not for clinical diagnosis or human use. The user assumes all responsibility for any consequences arising from misuse.
Ordering & Contact Information
To order this Rabbit Type I Collagen (Col‑I) ELISA Kit, or to inquire about volume discounts, custom development, and format selection, please contact our sales team.
