Beyond the broad consequences of hemolysis, the value of a clean, well‑handled sample becomes even more apparent when you consider a specific, clinically significant analyte like complement C3. Complement C3 is the most abundant complement protein in serum and acts as the central hub of the immune defense network. It is the point where the classical, alternative, and lectin pathways converge, and upon proteolytic activation it is cleaved into the biologically active fragments C3a and C3b. Researchers rely on the complement C3 ELISA test kit to investigate how C3 levels correlate with key pathophysiological states. In inflammatory disease monitoring, C3 is an acute‑phase reactant — its serum concentration rises markedly in both acute and chronic inflammation, such as rheumatoid arthritis, making this ELISA test kit a practical tool for gauging inflammatory severity and treatment response. Conversely, in autoimmune diseases like systemic lupus erythematosus (SLE), excessive immune complex formation consumes complement, driving C3 levels down; here the ELISA test kit serves as a sensitive indicator of disease activity and a prognostic marker for renal or hematological flares. Beyond clinical correlation, the complement C3 ELISA test kit is widely used to dissect immune signaling pathways, helping to clarify how the complement system orchestrates host defense, tissue repair, and intracellular signal transduction. Because hemolysis can release erythrocyte‑derived proteases that degrade C3 and introduce peroxidase‑mimicking hemoglobin that falsely elevates the signal, only a rigorously validated ELISA test kit paired with meticulous sample preparation can deliver the reliable quantitative data these studies demand. At Yanda Bio, our complement C3 ELISA test kit is built to the same high standards of sensitivity, reproducibility, and matrix tolerance that define our entire portfolio — ensuring that when you control hemolysis at the source, your C3 data will accurately reflect the biology, not the artifact.
Product Overview: Why Measure Mouse Complement C3?
Complement C3 is the central component of the complement system, a key part of innate immunity. It is cleaved into active fragments (C3a, C3b) that opsonise pathogens, recruit immune cells, and trigger the membrane attack complex. In mouse models, C3 levels are critical for studying autoimmune diseases (e.g., lupus, glomerulonephritis), infectious diseases, and inflammatory disorders. Accurate quantification of Mouse Complement 3 (C3) is essential for:
- Translational research using murine models of complement‑mediated diseases
- Evaluating drug candidates targeting the complement cascade
- Investigating the role of C3 in inflammation, tissue injury, and regeneration
Yanda Bio’s Mouse Complement 3 (C3) ELISA Kit uses a validated sandwich immunoassay format to deliver precise, reproducible results. As a professional manufacturer with > 6,000 detectable targets and long‑standing collaborations with over 60 Chinese universities, we combine deep immunology expertise with rigorous quality control. Our kits serve both academic and pharmaceutical researchers, contributing to the growing complement C3 ELISA kits market.
Superior Quality Metrics
- Intra‑assay CV < 5 % — excellent within‑run precision
- Inter‑assay CV < 8 % — outstanding run‑to‑run consistency
- High specificity — no cross‑reactivity with mouse C4, C5, or other complement components
Kit Components: What’s Inside
| Component | 96‑Well Configuration | 48‑Well Configuration |
|---|---|---|
| Microplate (Pre‑coated) | 8 wells × 12 strips | 8 wells × 6 strips |
| Standards | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes |
| Sample Diluent | 6 mL | 3 mL |
| HRP‑Conjugated Detection Antibody | 6 mL | 3 mL |
| 20× Wash Buffer | 25 mL | 15 mL |
| Substrate A | 6 mL | 3 mL |
| Substrate B | 6 mL | 3 mL |
| Stop Solution | 6 mL | 3 mL |
| Plate Sealer | 2 sheets | 2 sheets |
| Manual | 1 copy | 1 copy |
Note: This kit uses a sandwich ELISA format with HRP‑labelled detection antibody.
Assay Principle: Sandwich ELISA
This kit employs a solid‑phase sandwich enzyme‑linked immunosorbent assay. The microplate is pre‑coated with a monoclonal antibody specific for Mouse Complement 3 (C3). Standards and samples are added, allowing C3 to bind to the immobilised antibody. After washing, a Horseradish Peroxidase (HRP)‑conjugated detection antibody is added, forming an antibody‑antigen‑antibody “sandwich”. Following a second wash, TMB substrate is added. The HRP enzyme catalyses a blue colour that turns yellow upon acidification. The optical density (OD) at 450 nm is directly proportional to the C3 concentration in the sample.
Sample Dilution Note: Samples are pre‑diluted 5‑fold during the procedure; multiply results by 5 to obtain actual concentrations.
Sample Collection & Preparation
| Sample Type | Collection & Processing |
|---|---|
| Serum | Use pyrogen/endotoxin‑free tubes. Avoid cell stimulation. Centrifuge at 3000 rpm for 10 minutes. |
| Plasma | Use EDTA, citrate or heparin anticoagulant. Centrifuge at 3000 rpm for 30 minutes. |
| Cell Supernatant | Centrifuge at 3000 rpm for 10 minutes to remove particles. |
| Tissue Homogenate | Homogenise tissue in PBS (1:9 w/v), centrifuge at 5000×g for 10 minutes at 2–8 °C. Collect supernatant. |
| Storage | Aliquot and store at –20 °C (≤ 1 month) or –80 °C (≤ 2 months). Avoid repeated freeze‑thaw cycles. |
Materials Required But Not Provided
- Microplate reader (450 nm)
- Precision pipettes and tips (0.5–10 μL, 2–20 μL, 20–200 μL, 200–1000 μL)
- 37 °C incubator or water bath
Important Notes for Assay Procedure
- Store the kit at 2–8 °C. Bring all reagents to room temperature for 20 minutes before use. Concentrated wash buffer may crystallise; dissolve crystals in a warm water bath.
- Return unused strips to the resealable bag with desiccant and store at 2–8 °C. Use promptly.
- The S0 standard (zero concentration) is the negative control/blank. Multiply final results by 5 (dilution factor).
- Strictly adhere to incubation times, volumes and sequence.
- Mix all liquid components thoroughly before use.
Reagent Preparation
20× Wash Buffer Dilution: Dilute 1 part 20× wash buffer with 19 parts distilled water to obtain 1× working wash buffer.
Washing Method
Manual: Aspirate liquid, fill each well with wash buffer, stand for 1 min, aspirate, and tap dry on absorbent paper. Repeat 5 times.
Automatic washer: Inject 350 μL per well, soak for 1 min, wash 5 times.
Assay Procedure
- Remove required strips after 20 min equilibration. Reseal unused strips and return to 4 °C.
- Standard wells: add 50 μL of each standard concentration.
- Sample wells: add 10 μL sample + 40 μL sample diluent. Blank wells: leave empty.
- Add 100 μL HRP‑conjugated detection antibody to all wells except blanks. Seal and incubate at 37 °C for 60 min.
- Aspirate, wash 5 times (manual or automatic).
- Add 50 μL Substrate A and 50 μL Substrate B to each well. Incubate at 37 °C in the dark for 15 min.
- Add 50 μL stop solution. Measure OD at 450 nm within 15 min.
Data Analysis
Plot standard curve: OD values (y‑axis) vs. standard concentrations (x‑axis) in Excel or curve‑fitting software (linear regression, R ≥ 0.9900). Calculate sample concentrations from the curve, then multiply by the dilution factor (5×).
Kit Performance Specifications
| Parameter | Performance |
|---|---|
| Accuracy | Standard curve linear regression coefficient (R) ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 1.0 μg/mL |
| Detection Range | 1.0 – 800 μg/mL |
| Specificity | No cross‑reactivity with mouse C3a, C3b, C4, C5, or other complement proteins |
| Intra‑assay CV | < 5 % |
| Inter‑assay CV | < 8 % |
| Storage | 2–8 °C, protect from light and moisture |
| Shelf Life | 6 months |
Best Practices for Recording ELISA Experiment Information
Accurate and detailed record‑keeping is essential for reproducibility and scientific progress. We recommend that every user document the following for each assay:
- Date of experiment and operator name
- Experiment objective and detection target (Mouse C3)
- Sample information: source, processing steps, storage conditions, dilution factors
- Kit lot number and expiration date
- Standard curve data: concentrations and corresponding OD values (raw and blank‑subtracted)
- Sample OD values and calculated concentrations
- Any deviations from the standard protocol
- Observations: unusual colour development, precipitate, bubbles, etc.
Keeping a clear, traceable lab notebook (digital or paper) helps with troubleshooting, data validation, and learning from both successful and failed experiments. Share your insights with colleagues to promote a culture of rigorous science.
Related Products (Recommended for Cross‑Species & Activation Studies)
Expand your complement research with these complementary assays:
- Human Complement C3 ELISA Kit — for clinical and translational studies
- Human Complement Fragment 3a (C3a) ELISA Kit — specifically measures the bioactive anaphylatoxin C3a, enabling detailed analysis of complement activation
Using mouse and human C3 kits together allows direct comparison of complement status across species in translational models.
Special Offer
Buy any three ELISA kits from Yanda Bio and enjoy free shipping. Combine Mouse C3, Human C3, and Human C3a kits to build a comprehensive complement research panel at exceptional value.
The Complement C3 ELISA Kits: Quality & Affordability
As the demand for reliable complement assays grows in both academic and pharmaceutical settings, Yanda Bio delivers industry‑leading performance at a competitive price point. Our kits are designed to meet the rigorous requirements of the complement C3 ELISA kits market, offering:
- Cost‑effective pricing without sacrificing sensitivity or specificity
- Rapid customisation for unique sample matrices or detection ranges
- Batch‑to‑batch consistency for longitudinal and multi‑centre studies
Choose Yanda Bio for your mouse complement C3 quantification needs and experience the difference that experience and quality make.
Comparison with Premium International Brands
Yanda Bio’s ELISA kits deliver performance equivalent to leading international brands at 40–60 % lower cost. Our direct‑to‑researcher model and efficient manufacturing eliminate unnecessary markups. With superior precision (CV < 5 % intra‑assay, < 8 % inter‑assay) and 3–7 day custom development, we offer an unbeatable combination of quality, speed and affordability.
Disclaimer
Strict adherence to the protocol is required. Yanda Bio is not responsible for results obtained from deviations. This product is for research use only; not for clinical diagnosis or human use. The user assumes all responsibility for any consequences arising from misuse.
Ordering & Contact Information
To order this Mouse Complement 3 (C3) ELISA Kit, or to inquire about volume discounts, custom development, and format selection, please contact our sales team.
