The Human 2,6-Fructose Diphosphate (2,6-FD) ELISA Kit offers precise quantitative measurement of this pivotal allosteric regulator in carbohydrate metabolism, enabling researchers to directly assess the balance between glycolysis and gluconeogenesis: 2,6-FD serves as the most potent activator of 6-phosphofructo-1-kinase (PFK-1, the rate-limiting glycolytic enzyme) and simultaneously acts as a strong inhibitor of fructose-1,6-bisphosphatase (FBPase-1, a key gluconeogenic enzyme), making cellular 2,6-FD concentration a reliable indicator of metabolic flux. Because intracellular 2,6-FD levels are dynamically governed by the kinase and phosphatase activities of bifunctional PFKFB family enzymes, this assay also functions as an indirect readout of those enzymes’ regulatory status. In disease research, the human 2,6-FD ELISA kit supports oncology studies examining the Warburg effect by revealing how 2,6-FD changes in the tumor microenvironment influence cancer cell proliferation and apoptosis. It is equally valuable in metabolic disease research, helping to characterize glucose metabolism dysregulation in liver and muscle tissues from models of diabetes and obesity. For drug discovery and target validation, the kit enables pharmacodynamic evaluation of compounds aimed at glycolytic regulators like PFKFB3, allowing scientists to monitor 2,6-FD concentration shifts in response to inhibitors or activators and verify target engagement.
Advanced Detection Technology for Superior Data Quality
To deliver exceptional accuracy and minimize assay interference, this kit employs a premium signal amplification system:
- High-Fidelity Biotinylated Antibody: Utilizes a recombinant monoclonal antibody with >95% purity for outstanding specificity and low cross-reactivity.
- Using a highly compatible Elisa plate to ensure antibodies bind to ELISA Plates
- Enhanced Signal Amplification: Incorporates a high-activity Streptavidin-HRP conjugate (≥500 U/mg). The powerful biotin-streptavidin interaction provides significant signal enhancement.
- Reduced Matrix Interference: This combined system is engineered to directly circumvent common matrix effects and background noise, resulting in cleaner signals, superior sensitivity (<1.0 ng/mL), and reliable quantification in complex biological samples.
Complete Specifications & Performance Data
Assay Performance Summary
ParameterSpecification
Principle One-Step Sandwich ELISA with Biotin-Streptavidin Amplification
Accuracy (Linearity) Correlation Coefficient R ≥ 0.9900
Sensitivity < 1.0 ng/mL
Detection Range 1.0 – 200 ng/mL
Specificity: No significant cross-reactivity with structural analogs
Precision Intra-assay & Inter-assay CV < 15%
Shelf Life & Storage 6 months at 2-8°C, protected from light & moisture
Standard Curve & Key Components
Standard Concentrations: 0, 5, 10, 20, 40, 80 ng/mL (or as per specific batch).
Kit Includes (96T / 48T formats):
- Pre-coated Microplate (8 / 4 strips)
- Standard Set
- Sample Diluent
- Biotinylated Detection Antibody
- Streptavidin-HRP Conjugate
- 20X Wash Buffer
- TMB Substrate A & B
- Stop Solution
- Plate Sealers & Full Protocol
Simplified Assay Workflow
Typical Procedure Outline:
- Preparation: Bring all reagents to room temperature. Prepare 1X Wash Buffer by diluting the 20X concentrate.
- Addition: Add 50 µL of standards or appropriately prepared samples to the wells.
- Detection & Incubation: Add 100 µL of the prepared detection complex (Biotin-Ab + SA-HRP). Incubate at 37°C for 60 minutes.
- Wash: Aspirate and wash each well 5 times with 1X Wash Buffer.
- Development: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes in the dark.
- Stop & Read: Add 50 µL Stop Solution. Measure the absorbance at 450 nm within 15 minutes.
Sample Note: Optimal sample preparation (e.g., deproteinization for tissue/cell lysates) is recommended. Please refer to the detailed manual for specific guidelines.
Relater Product
Human Adiponectin (ADPN) ELISA Kit-Quantitative determination of Human Adiponectin (ADPN / Accp30) concentration in serum, plasma, cell culture supernatants, and tissue homogenates.
Human CCL18 ELISA Kit– For Immune Regulation & Fibrosis Research.
Human CC Chemokine Ligand 12 (CCL12) ELISA Kit-For immunology and cancer studies.
Academic Collaboration Offer
Publish & Receive a Free Kit!
Yanda Bio is committed to supporting the scientific community. If you cite this Human 2,6-FD ELISA Kit in your publication and link to our product page, we will gladly provide you with one free standard ELISA kit of your choice (up to $120 value). Contact us after your paper’s acceptance to arrange this.
Ordering & Customized Solutions
Flexible Purchase Options
- Standard Purchase: $120 per kit. Ideal for individual research projects.
- Bulk/Wholesale Purchase: Contact us for pricing under $100 per kit. Designed for core facilities and high-volume studies.
- Custom ELISA Development: Our expert team can tailor assays for unique sensitivity needs, sample matrices, or specific analyte variants.
- Free Sample Testing Service: Validate performance with your samples at no cost before purchasing.
Why Partner with Yanda Bio?
As a certified manufacturer controlling production and quality, we ensure reliable, affordable reagents. Our collaborations with over 70 academic institutions underscore our commitment to supporting groundbreaking research with dependable tools.
Advance your research in carbohydrate metabolism and signaling. Contact us to purchase, request a quote, or learn more about our custom services and publication offer.
Research Applications & Support
Primary Applications (Research Use Only):
- Metabolic Disease Research: Investigate the role of F2,6BP in diabetes, insulin resistance, and metabolic syndrome.
- Cancer Metabolism: Study glycolytic flux and the Warburg effect in various tumor models.
- Hepatic & Endocrine Studies: Explore regulation of gluconeogenesis and glycolysis in liver physiology.
- Plant & Microbial Biochemistry (with appropriate validation).
Frequently Asked Questions (FAQ):
Q: What is the main advantage of this kit for measuring 2,6-FD?
A: The biotin-streptavidin amplification system is key. It provides a much stronger signal than conventional ELISAs, which is crucial for accurately detecting low levels of this potent metabolic regulator and overcoming interference in complex lysates.
Q: How do I qualify for the free kit publication offer?
A: Simply include a citation of our Human 2,6-FD ELISA Kit (Yanda Bio) in the methods section of your peer-reviewed article and provide a link to our website. Send us the published paper’s DOI or a preprint, and we will fulfill the offer.
Q: Can this kit be used for tissue samples from model organisms?
A: The antibodies are raised against the human target. Cross-reactivity with samples from other species (e.g., mouse, rat) is not guaranteed and requires validation by the researcher. Our custom service can potentially address such needs.
Q: Is this product for diagnostic use?
A: No. This kit is sold for Research Use Only (RUO). It is not intended for use in clinical diagnostic procedures.
Manufacturer’s Disclaimer
This product is for research applications only. It is not validated or approved for diagnostic, therapeutic, or clinical use. The end-user is responsible for ensuring proper assay conduct and compliance with all relevant regulations.
