Inflammatory Cytokine ELISA Kit
Inflammatory Cytokine ELISA Kit include interleukins (e.g., IL‑1β, IL‑6, IL‑10), tumor necrosis factors (TNF‑α), interferons (IFN‑γ), chemokines (e.g., MCP‑1), and many others. The YanDa Bio’s inflammatory factor ELISA kit is highly popular worldwide and is sold over 1,000 units annually in China.
Categories
Contact Us
- elisa@yandabiology.com
- +86 19078818340
Frequently Asked Questions
How to ensure that the ELISA kit will not be damaged during transportation.
We will use air transportation to ensure that the test kits are delivered to you within one week. During this period, we used EPS foam boxes to store the ELISA kits and used dry ice to ensure the temperature was below 20°C.
Could you possibly offer a trial pack?
If you need antibodies, we can offer a 10-microliter trial pack for you, but you will be responsible for the shipping cost. We do not provide the ELISA kit packaging, but we can offer you an ELISA kit at a price much lower than the market rate for your use.
What Payment Methods Do You Support?
Currently, we support credit card payment, bank transfer, and third-party payment platforms (such as Alipay,Paypal.WeChat Pay, etc. International business will be adjusted according to relevant regulations).
Could you offer ELISA testing service for me?
Yes.If you purchase our test kits, we will offer a free ELISA testing service. If you provide the test kits yourself, we will charge a testing fee of $50.
Could you offer the service of customizing ELISA kits?
Yes, we offer custom ELISA kit services. Both the test factors and the detection sensitivity of the ELISA kits can be customized.
Why is your product so cheap?
We are the original manufacturer and this is our first attempt to enter the international market. We hope to establish trust with you as quickly as possible. Believe me, choosing to trust us will be the best decision for you.
What Is an Inflammatory Cytokine ELISA Kit? Principle of Inflammatory Cytokine ELISA Detection Kit
Inflammatory cytokines are small signaling proteins secreted by immune cells (such as macrophages, T cells, and monocytes) and other cell types in response to infection, injury, or inflammation. They include interleukins (e.g., IL‑1β, IL‑6, IL‑10), tumor necrosis factors (TNF‑α), interferons (IFN‑γ), chemokines (e.g., MCP‑1), and many others. These molecules regulate the intensity and duration of inflammatory responses, coordinate immune cell recruitment, and play pivotal roles in diseases ranging from rheumatoid arthritis and sepsis to cancer and COVID‑19.
To measure the concentration of these key biomarkers in biological samples (serum, plasma, cell culture supernatant, tissue homogenates, etc.), researchers rely on a powerful and widely used tool: the inflammatory cytokine ELISA kit. In fact, this is the most common type of ELISA kit and the best‑selling category for many manufacturers, including Yanda Bio.
In this product overview, we will explain what an inflammatory cytokine ELISA kit is, how it works (the principle of the assay), and why choosing a reliable elisa diagnostic kit supplier like Yanda Bio can make all the difference in your research.
What Is an Inflammatory Cytokine ELISA Kit?
An inflammatory cytokine ELISA kit is a ready‑to‑use, standardized immunoassay designed to quantitatively or qualitatively detect specific inflammatory cytokines in a variety of sample matrices. These kits are indispensable for:
Immunology research – studying immune responses and inflammation mechanisms
Clinical diagnostics – monitoring disease activity (e.g., cytokine storm in severe infections)
Drug development – evaluating the anti‑inflammatory effects of new compounds
Vaccine studies – assessing cytokine profiles after immunization
Depending on the species of interest, you can find specialized kits such as a mouse inflammatory cytokine elisa kit, a rat inflammatory cytokine elisa kit, or kits for human, rabbit, and other animals. Each kit contains all necessary components: pre‑coated microplates, standards, detection antibodies, enzyme conjugates, wash buffer, substrate, and stop solution.
Principle of the Inflammatory Cytokine ELISA Detection Kit
The overwhelming majority of inflammatory cytokine ELISA kits employ the double‑antibody sandwich ELISA (also called sandwich immunoassay) format. This format offers high specificity and sensitivity because the target cytokine is captured between two antibodies that recognize different epitopes.
Step‑by‑Step Principle (Sandwich ELISA)
Step 1: Coating
A capture antibody specific for the target inflammatory cytokine (e.g., mouse IL‑6) is pre‑coated onto the wells of a 96‑well microplate. The plate is then blocked to prevent non‑specific binding.
Step 2: Sample and Standard Addition
The researcher adds either the sample (serum, plasma, cell culture supernatant, etc.) or a series of cytokine standards (known concentrations) into the wells. If the target cytokine is present, it binds to the capture antibody immobilized on the plate surface.
Step 3: Washing
Unbound substances are removed by washing the plate with a mild detergent solution (wash buffer). This step reduces background noise.
Step 4: Detection Antibody Binding
A biotinylated or enzyme‑linked detection antibody (recognizing a different epitope on the same cytokine) is added. This antibody binds to the captured cytokine, forming a “capture antibody‑cytokine‑detection antibody” sandwich complex.
Step 5: Enzyme Conjugate Addition
If the detection antibody is biotinylated, streptavidin conjugated to horseradish peroxidase (HRP) is added. If the detection antibody is already HRP‑labeled, this step is skipped. HRP is the most common enzyme used because of its high turnover rate and stability.
Step 6: Substrate Reaction
The HRP substrate, usually TMB (3,3’,5,5’‑tetramethylbenzidine) , is added. HRP catalyzes the conversion of TMB from colorless to a blue product. The reaction is allowed to proceed for a fixed time (usually 15–30 minutes) in the dark.
Step 7: Stopping and Reading
A stop solution (e.g., dilute sulfuric acid) is added, changing the color from blue to yellow. The absorbance is measured at 450 nm using a microplate reader. The color intensity is directly proportional to the amount of cytokine in the sample.
Step 8: Quantification
A standard curve is generated by plotting the absorbance of the standards against their known concentrations. The concentration of cytokine in each test sample is then calculated from this standard curve.
Note: Some inflammatory cytokine assays use a competitive ELISA format, but sandwich ELISA remains the gold standard for most interleukins and other mid‑sized proteins because of its excellent sensitivity and wide dynamic range.
Classification of Inflammatory Cytokine ELISA Kits
Inflammatory cytokine ELISA kits can be classified by several criteria:
By Detection Species
Human inflammatory cytokine ELISA kits – for clinical or human cell line research
Mouse inflammatory cytokine ELISA kit – widely used in preclinical animal models of inflammation, autoimmunity, and infection
Rat inflammatory cytokine elisa kit – essential for rat models of arthritis, neuroinflammation, and cardiovascular disease
Rabbit, monkey, porcine, and other species – available upon request or as custom development
At Yanda Bio, we offer thousands of targets across all these species. Whether you need a mouse inflammatory cytokine elisa kit for a sepsis study or a rat inflammatory cytokine elisa kit for a spinal cord injury model, we have you covered.
By Detection Format
Sandwich ELISA kits (most common for cytokines)
Competitive ELISA kits (for small molecule cytokines or when only one antibody is available)
High‑sensitivity ELISA kits (for low‑abundance cytokines like IL‑4 or IFN‑γ in unstimulated samples)
By Multiplexing Capability
Single‑plex (one cytokine per kit) – traditional and most accurate
Multiplex kits (multiple cytokines in one well) – for high‑throughput screening, though often with lower sensitivity
Why Choose Yanda Bio as Your ELISA Diagnostic Kit Supplier?
As a professional elisa diagnostic kit supplier, Yanda Bio has been serving researchers worldwide with high‑quality, affordable immunoassays. Our inflammatory cytokine ELISA kits stand out for several reasons:
Extensive Target Menu
We offer thousands of inflammatory cytokine targets, including IL‑1β, IL‑2, IL‑4, IL‑6, IL‑10, TNF‑α, IFN‑γ, MCP‑1, MIP‑1α, RANTES, and many more. For a complete list, please visit our [inflammatory cytokine ELISA kit product category page].
Rigorous Quality Control
Each lot is validated for linearity, sensitivity (low pg/mL range), intra‑ and inter‑assay precision (CV <10% and <15% respectively), and spike‑and‑recovery (80–120%). We provide a Certificate of Analysis with every kit.
Species Coverage
Human, mouse, rat, rabbit, and other species. Our mouse inflammatory cytokine elisa kit and rat inflammatory cytokine elisa kit are particularly popular among academic and pharmaceutical researchers.
Custom ELISA Development
Do not see your target or species? We offer custom ELISA kit development with a fast turnaround of only 5–7 business days from order to delivery. Simply contact our team with your requirements.
Unbeatable Value
Standard 96‑well kits start at just $120. Bulk orders receive significant discounts. We also provide free ELISA testing service (send us your samples, we run the assay for you).
Fast Shipping
Orders placed before 3:30 PM (China time) ship the same day; next‑day shipping for all other orders.
Recommended Products from Yanda Bio
Here are some of our best‑selling inflammatory cytokine ELISA kits:
Mouse IL‑6 ELISA Kit – sensitivity 2 pg/mL, range 7.8–500 pg/mL, ideal for sepsis and arthritis models.
Rat TNF‑α ELISA Kit – sensitivity 4 pg/mL, range 15.6–1000 pg/mL, widely used in neuroinflammation research.
Human IL‑1β High‑Sensitivity Kit – sensitivity 0.5 pg/mL, for detecting baseline levels in healthy serum.
Mouse IFN‑γ ELISA Kit – sensitivity 5 pg/mL, perfect for Th1 immune response studies.
For a full list, visit our [inflammatory cytokine ELISA kit collection]. If you need help choosing the right kit, read our [ELISA troubleshooting guide] or contact our technical support team.
Conclusion
Inflammatory cytokine ELISA kits are essential tools for understanding immune regulation, disease mechanisms, and therapeutic responses. Whether you work with human, mouse, or rat models, the sandwich ELISA principle provides accurate, reproducible quantification of these critical biomarkers.
As a trusted elisa diagnostic kit supplier, Yanda Bio is committed to delivering high‑performance inflammatory cytokine elisa kits – including the popular mouse inflammatory cytokine elisa kit and rat inflammatory cytokine elisa kit – at prices that fit your budget. With thousands of targets and a rapid custom service (5‑7 business days), you can focus on your science while we take care of the reagents.