Yanda Bio presents its precise Horse Calcitonin (CT) ELISA kit, a ready-to-use enzyme immunoassay for quantifying equine calcitonin in serum, plasma, cell culture supernatant, and tissue homogenates. Backed by our catalog of over 6,000 validated ELISA targets across human, mouse, rat, horse, rabbit, canine, bovine, chicken, duck, sheep, monkey, fish, insect, plant, and microbial species, Yanda Bio serves more than 60 universities and research institutes in China. Every kit we ship is characterized by high reproducibility, strong specificity, and excellent sensitivity—with custom sensitivity options available upon request. Take advantage of free shipping when you order any 3 kits, and leverage our complimentary elisa testing service: our skilled laboratory team will run the assay on your samples at no charge, record the full procedure on video, and deliver all raw data. For projects requiring ultra‑low detection limits, inquire about our NHS‑biotin‑amplified ELISA kits. We also offer immunogenicity elisa testing services for equine drug and vaccine development. This kit is validated for equine samples exclusively and must not be used with human specimens.
Key Features
- Specific for Equine Calcitonin: Antibodies target a horse-specific epitope; no cross-reactivity with calcitonin from other species.
- High Sensitivity & Wide Range: Minimum detectable concentration <0.1 ng/mL; quantitative range 0.1–16 ng/mL, supported by a six-point standard curve (0, 1, 2, 4, 8, 16 ng/mL).
- Recombinant Protein Standards: Standards are produced in a eukaryotic expression system, ensuring native-like conformation and immunoreactivity.
- Validated Sample Types: Equine serum, EDTA/citrate/heparin plasma, cell culture supernatant, tissue homogenates, cerebrospinal fluid (CSF), and synovial fluid.
- Comprehensive Tissue Preparation Protocol: Detailed guidance includes protease inhibitor use, endogenous peroxidase inactivation for high-background tissues, and low-temperature processing to preserve analyte integrity.
- Built-in 5× Sample Dilution: The assay automatically dilutes samples 5-fold in the well (10 µL sample + 40 µL diluent) — simply multiply the interpolated result by 5 to obtain the actual concentration.
- One-Step Sandwich ELISA: Simultaneous incubation of sample/standard and HRP-conjugated detection antibody; total assay time approximately 2 hours.
- Reliable Performance: Intra- and inter-assay CV <15%; standard curve linearity R ≥0.9900.
- Flexible Formats: 48-well and 96-well configurations with removable strips; up to 88 samples per 96-well plate (single wells).
- Storage & Stability: 6-month shelf life at 2–8°C, protected from light and moisture; cold-chain shipping.
Product Description
Calcitonin (CT) is a 32-amino-acid peptide hormone primarily secreted by the parafollicular C cells of the thyroid gland. In horses, calcitonin plays a key role in calcium and phosphate homeostasis by inhibiting osteoclast-mediated bone resorption and promoting renal calcium excretion. It is a critical biomarker in equine research on bone metabolism, calcium disorders, thyroid function, and endocrine pathophysiology.
The Yanda-HORSE-CT ELISA Kit uses a one-step double-antibody sandwich ELISA method. The microplate is pre-coated with an antibody specific for a horse calcitonin epitope. Standards and samples are incubated together with an HRP-labeled detection antibody. After washing, TMB substrate produces a colorimetric signal proportional to the calcitonin concentration. Absorbance is read at 450 nm, and sample concentrations are interpolated from the standard curve.
This kit is designed exclusively for research use and is not intended for clinical diagnostic applications.
Sample Collection & Preparation
Liquid Samples
| Sample Type | Procedure |
|---|---|
| Serum | Collect blood in pyrogen/endotoxin-free tubes without anticoagulant. Allow to clot, then centrifuge at 3000 rpm for 10 min. Separate serum promptly. |
| Plasma | Use EDTA, citrate, or heparin as anticoagulant. Centrifuge at 3000 rpm for 30 min; collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 3000 rpm for 10 min to remove cells and debris. |
| Cerebrospinal Fluid (CSF) | Collect in sterile tubes. Centrifuge at 1000 × g (or 2000–3000 rpm) for 20 min at 2–8°C. Carefully collect supernatant. For small volumes, use micro-dilution techniques; assay within 30 min after dilution to avoid degradation. |
| Synovial Fluid & Other Body Fluids | Treat similarly to CSF: collect in sterile containers, centrifuge at low temperature, and use clarified supernatant. If turbid or with precipitate, centrifuge or filter again before testing. |
Tissue Homogenates
General Principle: Work rapidly at low temperature (on ice) to prevent protein degradation and interference.
- Rinse: Perfuse or rinse fresh tissue with ice-cold PBS to remove residual blood.
- Weigh & Mince: Weigh tissue, cut into small pieces.
- Homogenize: Add ice-cold PBS or lysis buffer containing protease inhibitors (e.g., 1 mM PMSF or a protease inhibitor cocktail) at a 1:9 (w/v) ratio. Homogenize thoroughly on ice using a glass homogenizer or tissue grinder. For tough tissues, grinding in liquid nitrogen is recommended.
- Lysis & Centrifugation: Let the homogenate sit on ice for 20–30 min to complete lysis. Centrifuge at 5000 × g (or 12,000–15,000 rpm) for 5–20 min at 4°C. Collect the clear middle supernatant for testing.
- Endogenous Peroxidase Control: Tissues with high endogenous peroxidase activity (e.g., liver, kidney, pancreas) may produce false-positive signals. For such samples, treat the homogenate with 1% H₂O₂ for 15 min to inactivate endogenous peroxidases before assay.
Important Notes for All Samples:
- NaN₃ Prohibition: Do not use sodium azide as a preservative; it inhibits HRP and will cause false-negative results.
- Storage: If not tested immediately, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles. If precipitates form during storage, centrifuge again before use.
Critical Dilution Factor
The assay automatically applies a 5-fold dilution in the well (10 µL sample + 40 µL Sample Diluent). The concentration read from the standard curve represents the diluted sample. Multiply the obtained value by 5 to calculate the actual calcitonin concentration in the original specimen.
The S0 standard (0 ng/mL) serves as the blank/negative control.
Based on internal testing, calcitonin levels in normal equine serum/plasma typically fall within 1–4 ng/mL. For other sample types, we strongly recommend performing a pilot experiment to determine the optimal dilution factor.
Technical Specifications
| Parameter | Specification |
|---|---|
| Standard Concentrations (S0–S5) | 0, 1, 2, 4, 8, 16 ng/mL |
| Quantitative Range | 0.1 – 16 ng/mL |
| Analytical Sensitivity | < 0.1 ng/mL |
| Accuracy (Linearity) | Standard curve R ≥ 0.9900 |
| Precision | Intra-assay CV < 15%; Inter-assay CV < 15% |
| Specificity | Specific for horse calcitonin via a horse-specific epitope; no cross-reactivity with calcitonin from other species or other soluble structural analogues |
| Sample Dilution Factor | 5× (performed automatically in the well) |
| Shelf Life | 6 months |
| Storage & Transport | 2–8°C, protected from light and moisture; cold-chain shipping |
Representative Standard Curve
For detailed validation data, including spike-recovery and dilution linearity, please refer to the product instruction manual or contact technical support.
Kit Components
96-Well Format
| Component | Quantity | Notes |
|---|---|---|
| Microelisa Stripplate | 12 strips × 8 wells | Pre-coated with anti-equine CT antibody |
| Standard (0.3 mL/vial) | 6 vials | Ready-to-use liquid, 0–16 ng/mL |
| Sample Diluent | 6 mL | – |
| HRP-Conjugated Detection Antibody | 10 mL | Ready-to-use |
| 20× Wash Buffer | 25 mL | Dilute to 1× before use |
| Substrate A | 6 mL | – |
| Substrate B | 6 mL | – |
| Stop Solution | 6 mL | – |
| Plate Sealers | 2 sheets | – |
| Instruction Manual | 1 copy | – |
| Self-sealing Bag | 1 piece | For unused strip storage |
48-Well Format
| Component | Quantity | Notes |
|---|---|---|
| Microelisa Stripplate | 12 strips × 4 wells | Pre-coated, removable |
| Standard (0.3 mL/vial) | 6 vials | Ready-to-use liquid |
| Sample Diluent | 3 mL | – |
| HRP-Conjugated Detection Antibody | 5 mL | Ready-to-use |
| 20× Wash Buffer | 15 mL | Dilute to 1× |
| Substrate A | 3 mL | – |
| Substrate B | 3 mL | – |
| Stop Solution | 3 mL | – |
| Plate Sealers | 2 sheets | – |
| Instruction Manual | 1 copy | – |
| Self-sealing Bag | 1 piece | – |
Note: The 20× Wash Buffer may crystallize during cold storage. Warm in a water bath until crystals dissolve completely before dilution.
Assay Procedure Summary
- Equilibrate: Let the sealed foil pouch reach room temperature for 20 min. Remove required strips; reseal unused strips and store at 4°C.
- Add Standards & Samples:
- Standard wells: 50 µL of each standard.
- Sample wells: 10 µL sample + 40 µL Sample Diluent.
- Blank well(s): Leave empty.
- Add Detection Antibody: Add 100 µL of HRP-conjugated detection antibody to all wells except blank. Cover with plate sealer and incubate at 37°C for 60 minutes.
- Wash: Aspirate, blot dry. Fill each well with 1× Wash Buffer, let stand for 1 min, then discard. Repeat for 5 washes. Automated plate washer: 350 µL/well, 1-min soak, 5 cycles.
- Substrate: Add 50 µL each of Substrate A and Substrate B. Incubate in the dark at 37°C for 15 minutes.
- Stop & Read: Add 50 µL Stop Solution. Measure absorbance at 450 nm within 15 minutes.
Total assay time: approximately 2 hours.
Materials Required but Not Supplied:
- Microplate reader (450 nm)
- Adjustable pipettes and tips (0.5–1000 µL)
- 37°C incubator or water bath
- Distilled/deionized water
- Absorbent paper, wash bottle or automated plate washer
- Protease inhibitors (for tissue homogenization)
- 1% H₂O₂ (optional, for high-endogenous-peroxidase tissues)
Throughput
| Format | Max. Number of Samples (Single Wells) |
|---|---|
| 48-well | 39–40 samples |
| 96-well | 85–88 samples |
Includes 6 standard wells and 1–2 blank wells. For duplicate measurements, divide the sample number accordingly.
Expand Your Equine Calcium Metabolism Panel
For a comprehensive evaluation of calcium homeostasis and bone remodeling in horses, pair this horse calcitonin ELISA kit with the following related assays:
- Horse Parathyroid Hormone (PTH) ELISA Kit – the primary physiological antagonist of calcitonin; use both together to study the PTH‑calcitonin regulatory axis in equine calcium balance.
- Horse Osteocalcin (Bone Gla Protein) ELISA Kit – a marker of osteoblast activity and bone formation, ideal for monitoring bone turnover during growth, training, or metabolic bone disease.
- Horse IL-8 ELISA Kit – Equine Interleukin-8 Immunoassay for Chemotaxis & Inflammation Studies
- Horse IL-1β ELISA Kit – Quantify Equine Interleukin-1β with Confidence
Browse our full equine ELISA portfolio for additional bone metabolism markers, including vitamin D metabolites, bone‑specific alkaline phosphatase, and tartrate‑resistant acid phosphatase.
Frequently Asked Questions
Q: Why must I multiply my result by 5?
A: The assay dilutes the sample 5-fold directly in the well (10 µL sample + 40 µL diluent). The standard curve accounts for this dilution; the interpolated value represents the diluted concentration. Multiply by 5 to obtain the actual concentration in the original sample.
Q: Can I use this kit to measure calcitonin from other species?
A: No. The antibodies are specific for a horse calcitonin epitope and do not cross-react with calcitonin from other species. It is validated exclusively for equine samples.
Q: What is the expected calcitonin range in normal horse serum?
A: Based on internal data, normal equine serum/plasma levels typically fall between 1–4 ng/mL. We recommend running pilot experiments to verify the dilution scheme for your specific study.
Q: My tissue samples show unexpectedly high background. What should I do?
A: Some tissues (e.g., liver, kidney) contain high endogenous peroxidase activity. Treat the homogenate with 1% H₂O₂ for 15 minutes before the assay to inactivate these enzymes. Always include proper controls.
Q: Can I use CSF or synovial fluid samples?
A: Yes. CSF and synovial fluid are compatible with this kit. Process them as you would other body fluids — low-temperature centrifugation to remove particulates, and assay immediately after dilution if sample volume is limited. Avoid NaN₃ preservatives.
Q: How do I store my samples if I cannot test them immediately?
A: Aliquot samples in single-use volumes and store at -20°C or -80°C. Do not freeze-thaw repeatedly. Thaw completely at room temperature and mix before use.
Ordering & Contact Information
| Item | Details |
|---|---|
| Catalog Number | Yanda-HORSE-CT |
| Formats | 48-well / 96-well |
| Package Dimensions | 16 cm × 12.5 cm × 9.5 cm (neutral white box) |
| Shipping | Cold-chain transport (2–8°C), protected from light and moisture |
| Storage | 2–8°C; do not freeze |
| Shelf Life | 6 months |
| Intended Use | For research use only, not for diagnostic procedures |
Contact us
- WhatsApp: +86 198 3186 0669
- Email: songhawyble@gmail.com
For pricing, bulk orders, or customization inquiries, please reach out via the above channels.
Technical Documents & Support
- MSDS – Supplied with the kit or available on request.
- Technical Support – For detailed validation data, protocol assistance, or troubleshooting, contact us via WhatsApp or email.
Disclaimer
This product is for laboratory research use only and is not approved for clinical diagnosis, treatment, or prognosis in humans or animals. The manufacturer assumes no responsibility for consequences arising from improper use, protocol deviations, or unauthorized applications. Users are responsible for validating assay performance in their own sample matrices and experimental conditions.cision‑making. This equine calcitonin ELISA kit is validated for equine samples exclusively and must not be used with human specimens. Follow the provided protocol precisely; any deviations are the responsibility of the experimenter. Yanda Bio assumes no liability for consequences arising from improper use.
Yanda Bio – Delivering high‑quality equine ELISA kits, expert elisa testing service, and specialized immunogenicity elisa testing services to support your endocrine, metabolic, and skeletal research with reliable data and complete convenience.
